DEVELOPMENT OF A MICROBIAL PREPARATION BY USING A CHLORIMURON-ETHYL DEGRADING BACTERIUM, BACILLUS TEQUILENSIS JW18

A strain JW18 capable of highly degrading chlorimuron-ethyl was isolated from soil. JW18 was identified as Bacillus tequilensis based on 16S rRNA and analysis of morphology, physiological and biochemical characters. The optimal pH value and temperature were 7.0-7.5 and 37 degrees C. The 96h degradation rate of 50 mg/L chlorimuron-ethyl by JW18 was at about 99% in inorganic salt medium. The strain JW18 could tolerate 800 mg/L of chlorimuron-ethyl and showed a high tolerance to chlorimuron-ethyl. The salt resistance test demonstrated that JW18 grew well at NaC1 concentrations ranging from 0.5% to 8.0%. In addition, it was actualized to develop a new microbial preparation for dealing with chlorimuronethyl pollution. The commercial preparation (soil conditioner) added by strain JW18 has developed a new function for degrading chlorimuron-ethyl residue in soil, and the negative effect were not detected. Therefore, it was considered that strain JW18 could be applied for bioremediation of chlorimuron-ethyl pollution and development of microbial preparation. This study may provide theoretic basis and reference for prevention and control of pesticides pollution.