Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread

被引：106

作者：

Coombes, Janine L. ^{[1
]}

Charsar, Brittany A. ^{[1
]}

Han, Seong-Ji ^{[1
]}

Halkias, Joanna ^{[1
]}

Chan, Shiao Wei ^{[1
]}

Koshy, Anita A. ^{[2
,3
]}

Striepen, Boris ^{[4
,5
]}

Robey, Ellen A. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Div Immunol & Pathogenesis, Berkeley, CA 94720 USA

[2] Stanford Univ, Dept Med, Sch Med, Div Infect Dis, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Neurol, Sch Med, Stanford, CA 94305 USA

[4] Univ Georgia, Paul Coverdell Ctr, Ctr Trop & Emerging Global Dis, Athens, GA 30602 USA

[5] Univ Georgia, Paul Coverdell Ctr, Dept Cellular Biol, Athens, GA 30602 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 21期

基金：

英国惠康基金;

关键词：

neutrophil motility; dynamic imaging; gut; mucosal immunology; DENDRITIC CELLS; ORAL INFECTION; T-CELL; LISTERIA-MONOCYTOGENES; INFLAMMATORY MONOCYTES; PATHOGEN INTERACTIONS; IFN-GAMMA; DISSEMINATION; MIGRATION; SALMONELLA;

D O I：

10.1073/pnas.1220272110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Toxoplasma gondii infection occurs through the oral route, but we lack important information about how the parasite interacts with the host immune system in the intestine. We used two-photon laser-scanning microscopy in conjunction with a mouse model of oral T. gondii infection to address this issue. T. gondii established discrete foci of infection in the small intestine, eliciting the recruitment and transepithelial migration of neutrophils and inflammatory monocytes. Neutrophils accounted for a high proportion of actively invaded cells, and we provide evidence for a role for transmigrating neutrophils and other immune cells in the spread of T. gondii infection through the lumen of the intestine. Our data identify neutrophils as motile reservoirs of T. gondii infection and suggest a surprising retrograde pathway for parasite spread in the intestine.

引用

页码：E1913 / E1922

页数：10

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