MicroRNA-211, a direct negative regulator of CDC25B expression, inhibits triple-negative breast cancer cells' growth and migration

被引:30
作者
Song, Guo-qing [1 ]
Zhao, Yi [1 ]
机构
[1] China Med Univ, Shengjing Hosp, Dept Pancreat & Breast Surg, Shenyang 110004, Liaoning, Peoples R China
关键词
MicroRNA-211; CDC25B; CCNB1; FOXM1; Breast cancer; POOR-PROGNOSIS; UP-REGULATION; CARCINOMA; MELANOMA; FOXM1;
D O I
10.1007/s13277-015-3151-6
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The non-coding microRNAs (miRNAs) have tissue-and disease-specific expression patterns. Dysregulation of miRNAs has been associated with initiation and progression of oncogenesis in humans. The abnormal expression of CDC25B phosphatases detected in a number of tumors implies that their dysregulation is involved in malignant transformation. Using miRNAtarget prediction software, we found that miR-211 could target the 3'UTR sequence of CDC25B. To shed light on their roles of miR-211 in breast cancer, the expression of miR-211 was examined by real-time RT-PCR in breast cancer and normal tissues. MiR-211 is significantly downregulated in breast cancer. MiR-211 re-expression suppressed cell growth, cell cycle, migration, and invasion in triple-negative breast cancer (TNBC) cell line MDA-MB231. Luciferase expression from a reporter vector containing the CDC25B -3'UTR was decreased when this construct was transfected with miR-211. The over-expression of miR-211 suppressed the endogenous CDC25B protein level in TNBC cells. For the first time, we demonstrate that miRNA-211 is a direct negative regulator of CDC25B expression in TNBC cells, alters other related target proteins CCNB1 and FOXM1, and then inhibits breast cancer cells growth, migration, and invasion and lead G2/M arrest. The transcriptional loss of miR-211 and the resultant increase in CDC25B expression facilitate increased genomic instability at an early stage of tumor development.
引用
收藏
页码:5001 / 5009
页数:9
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