Pre-analytical method for metabolic profiling of plant cell cultures of Passiflora garckei

被引：15

作者：

Fraccaroli, Marica ^{[1
]}

Nicoletti, Silvia ^{[1
]}

Maltese, Federica ^{[2
]}

Choi, Young Hae ^{[2
]}

Guzzo, Flavia ^{[1
]}

Levi, Marisa ^{[1
]}

Verpoorte, Robert ^{[2
]}

机构：

[1] Univ Verona, Fac Math Phys & Nat Sci, Sci & Technol Dept, I-37100 Verona, Italy

[2] Leiden Univ, Inst Biol, Sect Metabol, Div Pharmacognosy, Leiden, Netherlands

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 11期

关键词：

metabolomics; nuclear magnetic resonance spectroscopy; Passiflora garckei cell culture; solid phase extraction;

D O I：

10.1007/s10529-008-9785-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparation method. Solid phase extraction (SPE) was employed to isolate the plant metabolites for nuclear magnetic resonance (NMR) analysis and to subsequently detect the differences between yeast extract elicited and control cells. Compared with previous results obtained by using a Sephadex LH-20 column, SPE coupled with NMR spectroscopy improves the analysis of aromatic compounds e. g.: trans-feruloyl derivatives and trans-coumaroyl derivatives. Moreover, it decreases the concentration of sugars that usually overlap with many plant metabolite signals.

引用

页码：2031 / 2036

页数：6

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