γ-tocopherol nebulization by a lipid aerosolization device improves pulmonary function in sheep with burn and smoke inhalation injury

被引：47

作者：

Hamahata, Atsumori ^{[1
]}

Enkhbaatar, Perenlei ^{[1
]}

Kraft, Edward R. ^{[1
]}

Lange, Matthias ^{[1
]}

Leonard, Scott W. ^{[2
]}

Traber, Maret G. ^{[2
]}

Cox, Robert A. ^{[1
]}

Schmalstieg, Frank C. ^{[1
]}

Hawkins, Hal K. ^{[1
]}

Whorton, Elbert B. ^{[1
]}

Horvath, Eszter M. ^{[3
]}

Szabo, Csaba ^{[3
]}

Traber, Lillian D. ^{[1
]}

Herndon, David N. ^{[4
]}

Traber, Daniel L. ^{[1
]}

机构：

[1] Univ Texas Med Branch, Galveston, TX 77555 USA

[2] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA

[3] Univ Med & Dent New Jersey, Newark, NJ USA

[4] Shriners Hosp Children, Galveston, TX 77555 USA

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 2008年 / 45卷 / 04期

关键词：

gamma-Tocopherol; Smoke inhalation injury; Burn; Acute lung injury; Ovine model; Free radicals;

D O I：

10.1016/j.freeradbiomed.2008.04.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fire accident victims who sustain both thermal injury to skin and smoke inhalation have gross evidence of systemic and pulmonary oxidant damage and acute lung injury. We hypothesized that gamma-tocopherol (gT), a reactive O-2 and N-2 scavenger, when delivered into the airway, would attenuate lung injury induced by burn and smoke inhalation. Acute lung injury was induced in chronically prepared, anesthetized sheep by 40% total burn surface area, third-degree skin burn and smoke insufflation (48 breaths of cotton smoke, < 40 degrees C). The study groups were: (1) Sham (not injured, flaxseed oil (FO)-nebulized, n = 6); (2) SA-neb (injured, saline-nebulized, n = 6); (3) FO-neb (injured, FO-nebulized, n = 6); and (4) gT+FO-neb (injured, gT and FO-nebulized, n = 6). Nebulization was started 1 h postinjury, and 24 ml of FO with or without gT (51 mg/ml) was delivered into airways over 47 h using our newly developed lipid aerosolization device (droplet size: 2.5-5 mu m). The burn- and smoke inhalation-induced pathological changes seen in the saline group were attenuated by FO nebulization; gT addition further improved pulmonary function. Pulmonary gT delivery along with a FO source may be a novel effective treatment strategy in management of patients with acute lung injury.

引用

页码：425 / 433

页数：9

共 68 条

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