Monitoring cell substrate interactions in exopolysaccharide-based films reinforced with chitin whiskers and starch nanoparticles used as cell substrates

被引：11

作者：

Rodriguez, Sol ^{[1
]}

Gatto, Francesca ^{[2
,3
]}

Pesce, Luca ^{[2
,4
]}

Canale, Claudio ^{[2
]}

Pompa, Pier Paolo ^{[2
]}

Bardi, Giuseppe ^{[2
]}

Lopez, Daniel ^{[5
]}

Torres, Fernando G. ^{[1
]}

机构：

[1] Pontificia Univ Catolica Peru, Dept Mech Engn, Ave Univ 1801, Lima 32, Peru

[2] IIT, Genoa, Italy

[3] Univ Salento, Dept Engn Innovat, Lecce, Italy

[4] Univ Genoa, Dept Phys, Genoa, Italy

[5] Inst Polymer Sci & Technol ICTP CSIC, Madrid, Spain

来源：

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS | 2018年 / 67卷 / 06期

关键词：

Cell attachment; cell-surface interaction; exopolysaccharides; Nostoc commune; CYANOBACTERIUM NOSTOC-COMMUNE; EXTRACELLULAR POLYSACCHARIDES; ADHESION; NANOCOMPOSITES; BIOSYNTHESIS; ROUGHNESS; GROWTH;

D O I：

10.1080/00914037.2017.1297942

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Exopolysaccharides (EPS) extracted from Nostoc commune cyanobacteria were used to prepare low-adhesion cell substrates for tumor-selective cell growth. The substrates were reinforced with chitin whiskers and starch nanoparticles and characterized using Fourier transform infrared spectroscopy, X-ray diffraction, and tensile tests. Two anchorage independent cells, green fluorescent protein-modified HeLa and THP-1 cells were seeded on the samples to assess their interaction with the composites. The results confirmed the cell-surface interaction of EPS-based composites. The mechanical properties of these films can be tailored by controlling the type and concentration of the reinforcement, which can be used in the design of different types of biomaterials.

引用

页码：333 / 339

页数：7

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