Reagentless recycling of pyruvate oxidase on a conducting polymer-modified electrode

被引：9
作者：
Dang, L. A. [1 ]
Haccoun, J. [1 ]
Piro, B. [1 ]
Pham, M. C. [1 ]
机构：
[1] Univ Paris 07, Lab ITODYS, CNRS, UMR 7086, F-75005 Paris, France
来源：
ELECTROCHIMICA ACTA | 2006年 / 51卷 / 19期
关键词：
conducting polymer; pyruvate oxidase; reagentless detection; quinone;
D O I：
10.1016/j.electacta.2005.11.007
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
The enzyme pyruvate oxidase (PyOD) covalently immobilized on an original conducting copolymer poly(5-hydroxy-1,4-naphthoquinone-co-5-hydroxy-3-thioacetic-1,4-naphthoquinone acid) can be recycled under anaerobic conditions, at +0.1 V versus SCE. It is first demonstrated that the quinone group is an efficient co-substrate for PyOD in homogeneous conditions, then this efficiency is preserved when the quinone group is embedded in the polymer structure. The copolymer remains efficient even in aerated media. The low working potential avoids side-oxidations of interfering species as ascorbic acid or salycilate. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：3934 / 3943
页数：10
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