Do Mesenchymal Stem Cells Modulate the Milieu of Reconstructed Bladder Wall?

被引:33
作者
Pokrywczynska, Marta [1 ]
Jundzill, Arkadiusz [1 ,2 ]
Bodnar, Magdalena [3 ]
Adamowicz, Jan [1 ]
Tworkiewicz, Jakub [1 ]
Szylberg, Lukasz [3 ]
Debski, Robert [5 ]
Marszalek, Andrzej [3 ,4 ]
Drewa, Tomasz [1 ,6 ,7 ]
机构
[1] Nicolaus Copernicus Univ Torun, Ludwik Rydygier Med Coll Bydgoszcz, Dept Tissue Engn, Karlowicza 24, PL-85092 Bydgoszcz, Poland
[2] Nicolaus Copernicus Univ Torun, Dept Gen & Vasc Surg, PL-85092 Bydgoszcz, Poland
[3] Nicolaus Copernicus Univ Torun, Ludwik Rydygier Med Coll Bydgoszcz, Dept Clin Pathomorphol, PL-85092 Bydgoszcz, Poland
[4] Poznan Univ Med Sci, Dept Pathol, Poznan, Poland
[5] Dept Pediat Hematol & Oncol, Bydgoszcz, Poland
[6] Nicolaus Copernicus Hosp, Dept Urol, Torun, Poland
[7] Inst Oncol, Dept Urol, Kielce, Poland
关键词
Bladder regeneration; Cytokines; Matrix metalloproteinases; Mesenchymal stem cells; Tissue engineering; GROWTH-FACTOR-BETA; REGENERATION; DIFFERENTIATION; UROTHELIUM; RECEPTORS; INDUCTION; PHENOTYPE; INJURY; MODEL;
D O I
10.1007/s00005-013-0249-7
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
To evaluate the mesenchymal stem cells (MSCs) influence on cytokines and matrix metalloproteinases (MMPs) expression in rat bladder wall regeneration. MSCs cultures from the bone marrow were established. Acellular matrices from the bladder submucosa were prepared. Bladders were reconstructed using cell-seeded (n = 5) and unseeded (n = 5) grafts. MSCs were injected into the bladder wall (n = 5), bladders were incised and MSCs were injected into the circulation (n = 5) or were left intact (n = 5). Animals were killed after 3 months. Bladder histology and immunohistochemical staining of IL-2, IL-4, IL-6, IL-10, TNF-alpha, TGF-beta 1, IFN-gamma, MMP-2, and MMP-9 were done. Bladders reconstructed with cell-seeded grafts mimicked native tissue, while unseeded grafts revealed shrinkage and morphological irregularities. There were no morphological changes in bladders of other groups. Different pattern of cytokine and MMP expression was observed. Increased expression of anti-inflammatory cytokines and MMPs in bladder promotes detrusor regeneration.
引用
收藏
页码:483 / 493
页数:11
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