Studies of DNA-binding and DNA-cutting mechanism of an azamacrocyclic cerium complex with carboxyl branch

被引：5

作者：

Feng, Fa-mei ^{[1
]}

Cai, Shu-lan ^{[1
]}

Liu, Fu-an ^{[1
]}

Xie, Jia-qing ^{[2
]}

机构：

[1] Sichuan Univ Sci & Engn, Coll Chem & Pharmaceut Engn, Zigong 643000, Sichuan, Peoples R China

[2] Chongqing Univ Technol, Coll Chem & Chem Engn, Chongqing 400054, Peoples R China

来源：

PROGRESS IN REACTION KINETICS AND MECHANISM | 2013年 / 38卷 / 03期

关键词：

DNA binding; DNA cutting; azamacrocyclic cerium complex; enzyme mimic; CLEAVAGE; HYDROLYSIS; PHOSPHODIESTER; COPPER(II); DINUCLEAR; DYNAMICS; RNA;

D O I：

10.3184/146867813X13738207456695

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Anazamacrocycliccompoundwithacarboxylbranch, 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclo-tetradecane-N'-acetic acid (L), and its cerium complex were synthesised and characterised. The mode of combination of the cerium complex with DNA was investigated by fluorescence and UV-Vis absorption spectroscopy. The cutting function of the cerium complex towards supercoiled DNA was studied by the gel electrophoresis method. The results show that metal complex can bind to the phosphate group of DNA double helix and promote the hydrolysis of the phosphodiester bond of supercoiled DNA (Form I); under certain conditions, supercoiled form DNA (Form I) was transformed into the nicked form DNA (Form II) under the strong cutting effect of the macrocyclic cerium complex; the cutting is completed by a hydrolysis mechanism.

引用

页码：283 / 294

页数：12

共 26 条

[1] NMR, relaxometric, and structural studies of the hydration and exchange dynamics of cationic lanthanide complexes of macrocyclic tetraamide ligands [J].

Aime, S ;

Barge, A ;

Bruce, JI ;

Botta, M ;

Howard, JAK ;

Moloney, JM ;

Parker, D ;

de Sousa, AS ;

Woods, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (24) :5762-5771

[2] Introduction to RNA/DNA cleavage [J].

Bashkin, JK .

CHEMICAL REVIEWS, 1998, 98 (03) :937-937

[3] Metal complex-DNA interactions: from transcription inhibition to photoactivated cleavage [J].

Boerner, LJK ;

Zaleski, JM .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2005, 9 (02) :135-144

[4] Gadolinium(III) chelates as MRI contrast agents: Structure, dynamics, and applications [J].

Caravan, P ;

Ellison, JJ ;

McMurry, TJ ;

Lauffer, RB .

CHEMICAL REVIEWS, 1999, 99 (09) :2293-2352

[5] Triisopropyltriazacyclononane copper(II): An efficient phosphodiester hydrolysis catalyst and DNA cleavage agent [J].

Deck, KM ;

Tseng, TA ;

Burstyn, JN .

INORGANIC CHEMISTRY, 2002, 41 (04) :669-677

[6] Comparing a mononuclear Zn(II) complex with hydrogen bond donors with a dinuclear Zn(II) complex for catalysing phosphate ester cleavage [J].

Feng, GQ ;

Mareque-Rivas, JC ;

Williams, NH .

CHEMICAL COMMUNICATIONS, 2006, (17) :1845-1847

[7] Lanthanide-mediated DNA hydrolysis [J].

Franklin, SJ .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (02) :201-208

[8] Glycine-alanine conjugated macrocyclic lanthanide ion complexes as artificial ribonucleases [J].

Gunnlaugsson, T ;

O'Brien, JE ;

Mulready, S .

TETRAHEDRON LETTERS, 2002, 43 (47) :8493-8497

[9] Interaction of bis-aryl functionalized molecules with nucleosides and nucleic acids [J].

Huang Yu ;

Liu JunLiang ;

Zhang Ji ;

Liu Qiang ;

Hou JiTing ;

Zhang Yu ;

Zhang DaWei ;

Lu QiaoSen ;

Chen ShanYong ;

Lin HongHui ;

Yu XiaoQi .

SCIENCE CHINA-CHEMISTRY, 2010, 53 (01) :103-112

[10] Cooperativity between metal ions in the cleavage of phosphate diesters and RNA by dinuclear Zn(II) catalysts [J].

Iranzo, O ;

Elmer, T ;

Richard, JP ;

Morrow, JR .

INORGANIC CHEMISTRY, 2003, 42 (24) :7737-7746

← 1 2 3 →