In vitro expression of Fas and CD40 and induction of apoptosis in human cystic fibrosis airway epithelial cells

被引:11
|
作者
Amsellem, C
Durieu, I
Chambe, MT
Peyrol, S
Pachéco, Y
机构
[1] Univ Lyon 1, Equipe Accueil 3091, Ctr Hosp Lyon Sud, F-69310 Pierre Benite, France
[2] RTH Laennec, Fac Med, Ctr Commun Microscopie Electron, F-69372 Lyon, France
关键词
cystic fibrosis; Fas; programmed cell death; tracheal cells;
D O I
10.1053/rmed.2001.1257
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Cystic fibrosis is characterized by a damaged airway epithelium with inflammation and chronic infection. The aim of this study was to investigate the process of apoptosis in this disease. To evaluate the effects of interferon gamma and the Fas apoptotic pathway on cystic fibrosis airway epithelial cells, we used immortalized cystic fibrosis (CFT-1 and CFT-2) and normal (NT-1) human tracheal epithelial cell lines. Cell death was determined using annexin-V/propidium iodide labeling and electron microscopy In vitro expression of Fas and CD40 surface antigens was analysed by immunofluorescence staining and flow cytometry Normal and cystic fibrosis cells constitutively express these antigens. CD40, but not Fas expression, was upregulated by interferon gamma. Treatment of interferon gamma-stimulated cells anti-Fas resulted in apoptosis for about 80% of CFT-2 (homozygous for DeltaF508 deletion) cells and for 35-40% of CFT-1 (heterozygous) or normal cells. Our results suggest that Fas may mediate apoptosis in cystic fibrosis airway epithelium.
引用
收藏
页码:244 / 249
页数:6
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