Ethylene inhibited aflatoxin biosynthesis is due to oxidative stress alleviation and related to glutathione redox state changes in Aspergillus flavus

被引：38

作者：

Huang, J. -Q ^{[1
]}

Jiang, H. -F. ^{[1
]}

Zhou, Y. -Q. ^{[1
]}

Lei, Y. ^{[1
]}

Wang, S. -Y. ^{[1
]}

Liao, B. -S. ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Oil Crops Res Inst, Peanut Res Grp, Key Lab Biol Sci Oil Crops,Minist Agr, Wuhan 430062, Peoples R China

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2009年 / 130卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Aflatoxin; Ethylene; Aspergillus flavus; Oxidative stress; Redox state; PARASITICUS; GROWTH; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE; B-1; PURIFICATION; ANTIOXIDANTS; EXPRESSION; REDUCTASE; PEROXIDE; SEQUENCE;

D O I：

10.1016/j.ijfoodmicro.2008.12.027

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The effect of 2-chloroethyl phosphoric acid (CEPA) on aflatoxin biosynthesis, the expression of aflatoxin biosynthetic genes, reactive oxygen species (ROS) formation, cellular redox status, and enzymes involved in glutathione consumption and regeneration in Aspergillus flavus was investigated. The results demonstrated that CEPA dose dependently inhibited aflatoxin B, production. The expression of two typical genes involved in aflatoxin biosynthesis, aflR and aflD, was reduced after CEPA treatment at 7 d. Meanwhile, CEPA significantly reduced ROS production and thiobarbituric acid reactive substances (TBARS), increased the ratio of reduced glutathione (GSH) and oxidized glutathione (GSSG) at 5, 6 and 7 d. The activities of glutathione peroxidase (GPx), glutathione reductase (GR), as well as glucose-6-phosphate dehydrogerase (G6PDH) were significantly inhibited after CEPA treatment at 5, 6 and 7 d. The present study suggested that ethylene reduced aflatoxin production is due to oxidative stress alleviation of fungal cells and is related to glutathione redox state changes. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：17 / 21

页数：5

共 41 条

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