Aberrant Peripheral Immune Function in a Good Syndrome Patient

被引:8
作者
Chen, Xian [1 ,2 ]
Zhang, Jie-xin [1 ,2 ]
Shang, Wen-wen [1 ,2 ]
Xie, Wei-ping [3 ]
Jin, Shu-xian [3 ]
Wang, Fang [1 ,2 ]
机构
[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Lab Med, Nanjing 210029, Jiangsu, Peoples R China
[2] Natl Key Clin Dept Lab Med, Nanjing 210029, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, Nanjing 210029, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
REGULATORY T-CELLS; THYMOMA; CANCER;
D O I
10.1155/2018/6212410
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Good's syndrome (GS) is often accompanied by recurrent respiratory infections and chronic diarrhea. The main purpose was to evaluate the peripheral immune status of a GS patient after thymoma resection. Twenty healthy volunteers were recruited as healthy controls (HCs). Flow cytometry was applied to determine the proportions of circuiting CD4(+) T cells, CD8(+) T cells, gamma delta T cells, and regulatory T (Treg) cells in our GS patient. We also examined the proliferation capability of ex vivo CD4(+) T cells and detected the levels of cytokines interferon- (IFN-) gamma and interleukin-17A secreted by ex vivo immune cells from this GS patient. Compared with healthy control subjects, this GS patient had fewer B cells, an inverted ratio of CD4(+)/CD8(+) cells, and more Treg cells in his peripheral blood. Additionally, the patient's V delta 2 T cell levels were significantly decreased despite having a normal percentage of gamma delta T cells. Ex vivo peripheral CD4(+) T cells from the patient showed insufficient proliferation and division potential as well as excessive expression of PD-1. Moreover, IFN-gamma was predominantly derived from CD8(+) T cells in this GS patient, rather than from CD4(+) T cells and gamma delta T cells. This GS patient had impaired T and B cell immunological alternations and cytokine disruptions after thymectomy. Detailed research should focus on therapies that can adjust the immune status in such patients for a better outcome.
引用
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页数:10
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