Prediction of new vaccine targets in the core genome of Corynebacterium pseudotuberculosis through omits approaches and reverse vaccinology

被引：22

作者：

Araujo, Carlos Leonardo ^{[1
]}

Alves, Jorianne ^{[1
]}

Nogueira, Wylerson ^{[2
]}

Pereira, Lino Cesar ^{[1
]}

Gomide, Anne Cybelle ^{[2
]}

Ramos, Rommel ^{[1
]}

Azevedo, Vasco ^{[2
]}

Silva, Artur ^{[1
]}

Folador, Adriana ^{[1
]}

机构：

[1] Fed Univ Para, Ctr Genom & Syst Biol, Lab Genom & Bioinformat, Belem, Para, Brazil

[2] Univ Fed Minas Gerais, Dept Gen Biol, Belo Horizonte, MG, Brazil

来源：

GENE | 2019年 / 702卷

关键词：

Corynebacterium pseudotuberculosis; Core genome; Omics approaches; Reverse vaccinology; ESCHERICHIA-COLI; MYCOBACTERIUM-TUBERCULOSIS; DIHYDROXYACETONE KINASE; SIGNAL-TRANSDUCTION; SUBSTRATE-BINDING; PAN-GENOMES; GENE; EXPRESSION; VIRULENCE; IDENTIFICATION;

D O I：

10.1016/j.gene.2019.03.049

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Corynebacterium pseudotuberculosis is the etiologic agent of veterinary relevance diseases, such as caseous lymphadenitis, affecting different animal species causing damage to the global agribusiness. So far, there are no completely effective treatment methods to overcome the impacts caused by this pathogen. Several genomes of the species are deposited on public databases, allowing the execution of studies related to the pan-genomic approach. In this study, we used an integrated in silico workflow to prospect novel putative targets using the core genome, a set of shared genes among 65 C. pseudotuberculosis strains. Subsequently, through RNA-Seq data of the same abiotic stresses in two strains, we selected only induced genes to compose the reverse vaccinology workflow based in two different strategies. Our results predicted six probable antigens in both analysis, which indicates that they have a strong potential to be used in further studies as vaccine targets against this bacterium.

引用

页码：36 / 45

页数：10

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