The Renoprotective Effect of Bone Marrow-Derived Endothelial Progenitor Cell Transplantation on Acute Ischemia-Reperfusion Injury in Rats

被引：16
作者：
Chen, B. [1 ]
Bo, C. -J. [2 ]
Jia, R. -P. [2 ]
Liu, H. [2 ]
Wu, R. [2 ]
Wu, J. [2 ]
Ge, Y. -Z. [2 ]
Teng, G. -J. [1 ]
机构：
[1] Southeast Univ, Sch Med, Dept Urol, Nanjing, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Nanjing Hosp 1, Dept Urol & Renal Transplantat, Nanjing 210006, Jiangsu, Peoples R China
来源：
TRANSPLANTATION PROCEEDINGS | 2013年 / 45卷 / 05期
基金：
中国国家自然科学基金;
关键词：
REPAIR; ANGIOGENESIS;
D O I：
10.1016/j.transproceed.2013.01.096
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Objective. To investigate the renoprotective effects of exogenous endothelial progenitor cells (EPCs) on acute renal ischemia-reperfusion (I/R) injury in rats. Methods. EPCs were collected by in vitro culture of mononuclear cells derived from rat bone marrow. The EPC labeling was performed using chloromethyl-benzamidodialkylcarbocyanine (CM-Dil). Sprague-Dawley rats were equally randomized into an I/R, an EPC, and a control group. We evaluated blood urea nitrogen (BUN) and serum creatinine (Scr), kidney morphology, apoptosis and microvessel density. EPC homing into I/R injured kidneys was observed using a fluorescence microscope. Results. After EPC transplantation, CM-Dil-labeled EPCs were noted at the corticomedullary junction of injured kidneys. The levels of BUN and Scr were markedly lower among the EPC than the I/R group (P < .05). The histopathologic score was higher in the I/R than the EPC group (P < .05). Apoptosis of tubular epithelial cells was substantially reduced among EPC-treated rats (P < .01). In addition, more CD34(+) microvessels were documented among the EPC than the other two groups (P < .01). The expression levels of vascular endothelial growth factor (VEGF) were also increased greatly in the EPC group (P < .05). Conclusion. Transplanted exogenous EPCs exert protective effects on renal function by maintaining the integrity of peritubular capillaries after I/R injury.
引用
收藏
页码：2034 / 2039
页数：6
相关论文
共 23 条
[11]  
Kribben A, 2003, DEUT MED WOCHENSCHR, V128, P1231
[12]  
Kudo FA, 2003, INT ANGIOL, V22, P344
[13]   Mobilized Human Hematopoietic Stem/Progenitor Cells Promote Kidney Repair After Ischemia/Reperfusion Injury [J].
Li, Bing ;
Cohen, Amy ;
Hudson, Thomas E. ;
Motlagh, Delara ;
Amrani, David L. ;
Duffield, Jeremy S. .
CIRCULATION, 2010, 121 (20) :2211-2220
[14]   Essential but differential role for CXCR4 and CXCR7 in the therapeutic homing of human renal progenitor cells [J].
Mazzinghi, Benedetta ;
Ronconi, Elisa ;
Lazzeri, Elena ;
Sagrinati, Costanza ;
Ballerini, Lara ;
Angelotti, Maria Lucia ;
Parente, Eliana ;
Mancina, Rosa ;
Netti, Giuseppe Stefano ;
Becherucci, Francesca ;
Gacci, Mauro ;
Carini, Marco ;
Gesualdo, Loreto ;
Rotondi, Mario ;
Maggi, Enrico ;
Lasagni, Laura ;
Serio, Mario ;
Romagnani, Sergio ;
Romagnani, Paola .
JOURNAL OF EXPERIMENTAL MEDICINE, 2008, 205 (02) :479-490
[15]   A subpopulation of endothelial progenitor cells with low aldehyde dehydrogenase activity attenuates acute ischemic brain injury in rats [J].
Nakamura, Kazuhiro ;
Tsurushima, Hideo ;
Marushima, Aiki ;
Nagano, Masumi ;
Yamashita, Toshiharu ;
Suzuki, Kensuke ;
Ohneda, Osamu ;
Matsumura, Akira .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2012, 418 (01) :87-92
[16]   Dynamics of mobilization and homing of endothelial progenitor cells after acute renal ischemia: modulation by ischemic preconditioning [J].
Patschan, Daniel ;
Krupincza, Krystina ;
Patschan, Susann ;
Zhang, Zhongtao ;
Hamby, Carl ;
Goligorsky, Michael S. .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2006, 291 (01) :F176-F185
[17]   Identification of vascular progenitor cells in pulmonary arteries of patients with chronic obstructive pulmonary disease [J].
Peinado, VI ;
Ramírez, J ;
Roca, J ;
Rodriguez-Roisin, R ;
Barberà, JA .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2006, 34 (03) :257-263
[18]   Myocardial regeneration by transplantation of modified endothelial progenitor cells expressing SDF-1 in a rat model [J].
Schuh, Alexander ;
Kroh, Andreas ;
Konschalla, Simone ;
Liehn, Elisa A. ;
Sobota, Radoslav M. ;
Al Biessen, Erik ;
Bot, Ilze ;
Taha, Soenmez Tolga ;
Schober, Andreas ;
Marx, Nikolaus ;
Weber, Christian ;
Sasse, Alexander .
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2012, 16 (10) :2311-2320
[19]   Change in mitochondrial membrane potential is the key mechanism in early warm hepatic ischemia-reperfusion injury [J].
Sun, CK ;
Zhang, XY ;
Sheard, PW ;
Mabuchi, A ;
Wheatley, AM .
MICROVASCULAR RESEARCH, 2005, 70 (1-2) :102-110
[20]   Pretreatment of Endothelial Progenitor Cells With Osteopontin Enhances Cell Therapy for Peripheral Vascular Disease [J].
Vaughan, E. E. ;
Liew, A. ;
Mashayekhi, K. ;
Dockery, P. ;
McDermott, J. ;
Kealy, B. ;
Flynn, A. ;
Duffy, A. ;
Coleman, C. ;
O'Regan, A. ;
Barry, F. P. ;
O'Brien, T. .
CELL TRANSPLANTATION, 2012, 21 (06) :1095-1107
123