The Renoprotective Effect of Bone Marrow-Derived Endothelial Progenitor Cell Transplantation on Acute Ischemia-Reperfusion Injury in Rats

被引：16
作者：
Chen, B. [1 ]
Bo, C. -J. [2 ]
Jia, R. -P. [2 ]
Liu, H. [2 ]
Wu, R. [2 ]
Wu, J. [2 ]
Ge, Y. -Z. [2 ]
Teng, G. -J. [1 ]
机构：
[1] Southeast Univ, Sch Med, Dept Urol, Nanjing, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Nanjing Hosp 1, Dept Urol & Renal Transplantat, Nanjing 210006, Jiangsu, Peoples R China
来源：
TRANSPLANTATION PROCEEDINGS | 2013年 / 45卷 / 05期
基金：
中国国家自然科学基金;
关键词：
REPAIR; ANGIOGENESIS;
D O I：
10.1016/j.transproceed.2013.01.096
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Objective. To investigate the renoprotective effects of exogenous endothelial progenitor cells (EPCs) on acute renal ischemia-reperfusion (I/R) injury in rats. Methods. EPCs were collected by in vitro culture of mononuclear cells derived from rat bone marrow. The EPC labeling was performed using chloromethyl-benzamidodialkylcarbocyanine (CM-Dil). Sprague-Dawley rats were equally randomized into an I/R, an EPC, and a control group. We evaluated blood urea nitrogen (BUN) and serum creatinine (Scr), kidney morphology, apoptosis and microvessel density. EPC homing into I/R injured kidneys was observed using a fluorescence microscope. Results. After EPC transplantation, CM-Dil-labeled EPCs were noted at the corticomedullary junction of injured kidneys. The levels of BUN and Scr were markedly lower among the EPC than the I/R group (P < .05). The histopathologic score was higher in the I/R than the EPC group (P < .05). Apoptosis of tubular epithelial cells was substantially reduced among EPC-treated rats (P < .01). In addition, more CD34(+) microvessels were documented among the EPC than the other two groups (P < .01). The expression levels of vascular endothelial growth factor (VEGF) were also increased greatly in the EPC group (P < .05). Conclusion. Transplanted exogenous EPCs exert protective effects on renal function by maintaining the integrity of peritubular capillaries after I/R injury.
引用
收藏
页码：2034 / 2039
页数：6
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