Enatiomeric separation of branched fatty acids after conversion with trans-2-(2,3-anthracenedicarboximido)cyclohexanol, a highly sensitive chiral fluorescent conversion reagent

被引：33
作者：
Akasaka, K [1 ]
Ohrui, H [1 ]
机构：
[1] Tohoku Univ, Grad Sch Agr Sci, Div Appl Life Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 07期
关键词：
enantiomeric separation; chiral branched fatty acid; chiral fluorescence conversion reagent; high-performance liquid chromatography;
D O I：
10.1271/bbb.63.1209
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
(1R, 2R)-2-(2,3-Anthracenedicarboximido)cyclohexanol was synthesized as a highly sensitive chiral fluorescent conversion reagent. The diastereomeric derivatives of chiral branched fatty acids that had methyl ethyl chirality from the 2 to 12 position were separated into 2 peaks by reversed-phase HPLC and detected at the 10(-15) mole level by fluorometry.
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页码：1209 / 1215
页数：7
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