Enatiomeric separation of branched fatty acids after conversion with trans-2-(2,3-anthracenedicarboximido)cyclohexanol, a highly sensitive chiral fluorescent conversion reagent

被引：33

作者：

Akasaka, K ^{[1
]}

Ohrui, H ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Div Appl Life Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 07期

关键词：

enantiomeric separation; chiral branched fatty acid; chiral fluorescence conversion reagent; high-performance liquid chromatography;

D O I：

10.1271/bbb.63.1209

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(1R, 2R)-2-(2,3-Anthracenedicarboximido)cyclohexanol was synthesized as a highly sensitive chiral fluorescent conversion reagent. The diastereomeric derivatives of chiral branched fatty acids that had methyl ethyl chirality from the 2 to 12 position were separated into 2 peaks by reversed-phase HPLC and detected at the 10(-15) mole level by fluorometry.

引用

页码：1209 / 1215

页数：7

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