Oxidative stress on mitochondria and cell membrane of cultured rat hepatocytes and perfused liver exposed to ethanol

被引：99

作者：

Kurose, I ^{[1
]}

Higuchi, H ^{[1
]}

Kato, S ^{[1
]}

Miura, S ^{[1
]}

Watanabe, N ^{[1
]}

Kamegaya, Y ^{[1
]}

Tomita, K ^{[1
]}

Takaishi, M ^{[1
]}

Horie, Y ^{[1
]}

Fukuda, M ^{[1
]}

Mizukami, K ^{[1
]}

Ishii, H ^{[1
]}

机构：

[1] KEIO UNIV, SCH MED, DEPT INTERNAL MED, SHINJUKU KU, TOKYO 160, JAPAN

来源：

GASTROENTEROLOGY | 1997年 / 112卷 / 04期

关键词：

D O I：

10.1016/S0016-5085(97)70147-1

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background & Aims: The precise pathogenic significance of oxidative injury in the evolution of alcohol-induced liver disease is still obscure. The present report was designed to investigate whether ethanol alters the production of active oxidants and biological activities of hepatocytes. Methods: The following parameters in rat hepatocytes were investigated by using fluorescence probes in vitro and ex vivo: (1) mitochondrial membrane potential and membrane permeability transition, (2) oxygen radicals generation, (3) membrane barrier function, and (4) glutathione level. Results: Ethanol (50 mmol/L) increased oxidative stress in hepatocytes and subsequently induced an increased mitochondrial permeability transition and a decreased membrane potential. These ethanol-induced alterations were attenuated by an inhibitor of alcohol dehydrogenase and an intracellular oxidant scavenger, whereas they were enhanced by diethyl maleic acid, a glutathione depletor. Ethanol plus diethyl maleic acid but not ethanol alone increased the number of hepatocytes with membrane barrier dysfunction. A continuous infusion of ethanol (50 mmol/L) increased oxidative stress and decreased mitochondrial membrane potential in the pericentral area of isolated perfused rat liver. Conclusions: Active oxidants generated during ethanol metabolism increase mitochondrial permeability transition and modulate mitochondrial energy synthesis in hepatocytes. Reduction of glutathione level enhances mitochondrial dysfunction and impairs membrane barrier function of hepatocytes.

引用

页码：1331 / 1343

页数：13

共 49 条

[21] CONJUGATION OF ACETALDEHYDE WITH CYSTEINYLGLYCINE, THE FIRST METABOLITE IN GLUTATHIONE BREAKDOWN BY GAMMA-GLUTAMYL-TRANSPEPTIDASE [J].

KERA, Y ;

KIRIYAMA, T ;

KOMURA, S .

AGENTS AND ACTIONS, 1985, 17 (01) :48-52

[22]

KOCAKTOKER N, 1985, PHARMACOL RES COMMUN, V17, P233

[23]

KONO Y, 1982, J BIOL CHEM, V257, P5751

[24] MICROVASCULAR RESPONSES TO INHIBITION OF NITRIC-OXIDE PRODUCTION - ROLE OF ACTIVE OXIDANTS [J].

KUROSE, I ;

WOLF, R ;

GRISHAM, MB ;

AW, TY ;

SPECIAN, RD ;

GRANGER, DN .

CIRCULATION RESEARCH, 1995, 76 (01) :30-39

[25]

KUROSE I, 1993, HEPATOLOGY, V18, P380, DOI 10.1016/0270-9139(93)90022-F

[26]

KUROSE I, 1993, CANCER RES, V53, P2676

[27] NITRIC-OXIDE MEDIATES MITOCHONDRIAL DYSFUNCTION IN HEPATOMA-CELLS INDUCED BY NONACTIVATED KUPFFER CELLS - EVIDENCE IMPLICATING ICAM-1-DEPENDENT PROCESS [J].

KUROSE, I ;

EBINUMA, H ;

HIGUCHI, H ;

YONEI, Y ;

SAITO, H ;

KATO, S ;

MIURA, S ;

ISHII, H .

JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 1995, 10 :S68-S71

[28] KUPFFER CELL-MEDIATED OXIDATIVE STRESS ON COLON CANCER CELL-LINE VISUALIZED BY DIGITAL IMAGING FLUORESCENCE MICROSCOPY [J].

KUROSE, I ;

SAITO, H ;

SUEMATSU, M ;

FUKUMURA, D ;

MIURA, S ;

MORIZANE, T ;

TSUCHIYA, M .

CANCER LETTERS, 1991, 59 (03) :201-209

[29] BLEBBING, FREE CA-2+ AND MITOCHONDRIAL-MEMBRANE POTENTIAL PRECEDING CELL-DEATH IN HEPATOCYTES [J].

LEMASTERS, JJ ;

DIGUISEPPI, J ;

NIEMINEN, AL ;

HERMAN, B .

NATURE, 1987, 325 (6099) :78-81

[30] LIPID HYDROPEROXIDE-INDUCED MITOCHONDRIAL DYSFUNCTION FOLLOWING ACUTE ETHANOL INTOXICATION IN RATS - THE CRITICAL ROLE FOR MITOCHONDRIAL REDUCED GLUTATHIONE [J].

MASINI, A ;

CECCARELLI, D ;

GALLESI, D ;

GIOVANNINI, F ;

TRENTI, T .

BIOCHEMICAL PHARMACOLOGY, 1994, 47 (02) :217-224

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