Anti-inflammatory and anti-oxidative effect of curcumin in connective tissue type mast cell

Curcumin in turmeric from rhizome of plant Curcuma longa was investigated on its anti-inflammatory effect with canine cutaneous mastocytoma mast cells. Though the degranulation induced by neuropeptide substance P, specific mast cell secregtagogue Compound 48/80 and immunoglobulin IgG were reached above 10%, curcumin reduced the release below 4.6% at 10 mu M without cytotoxicity. Inhibitory effect of curcumin on [Ca2+]i elevation was also confirmed. ROS induced by Compound 48/80 were evaluated with dichlorodihydrofluorescein diacetate (DCFH-DA), and were lowered by curcumin in dose dependent manner. Cell membrane lipid peroxidation was monitored with diphenyl-1-pyrenylphosphine (DPPP). Curcumin decreased cell membrane lipid peroxidation induced by arachidonic acid in the concentration above 0.01 mu M. Curcumin also inhibited the protein tyrosine- and threonine-phosphorylations around 33 and 45 kDa, respectively. Taking these findings into consideration with the inhibitory effects on connective tissue type mast cell, there is a potential use to ameliorate inflammation-related chronic diseases. (C) 2013 Elsevier Ltd. All rights reserved.