The AlkB Family of Fe(II)/α-Ketoglutarate-dependent Dioxygenases: Repairing Nucleic Acid Alkylation Damage and Beyond

被引：305

作者：

Fedeles, Bogdan I.

Singh, Vipender

Delaney, James C.

Li, Deyu

Essigmann, John M.

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

[2] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

[3] MIT, Ctr Environm Hlth Sci, Cambridge, MA 02139 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2015年 / 290卷 / 34期

基金：

美国国家卫生研究院;

关键词：

RESPONSE PROTEIN ALKB; AP LYASE ACTIVITY; ESCHERICHIA-COLI; DNA-REPAIR; OXIDATIVE DEMETHYLATION; TRANSFER-RNA; CRYSTAL-STRUCTURES; ENZYME ALKB; IN-VIVO; 2-OXOGLUTARATE-DEPENDENT OXYGENASES;

D O I：

10.1074/jbc.R115.656462

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The AlkB family of Fe(II)- and alpha-ketoglutarate-dependent dioxygenases is a class of ubiquitous direct reversal DNA repair enzymes that remove alkyl adducts from nucleobases by oxidative dealkylation. The prototypical and homonymous family member is an Escherichia coli "adaptive response" protein that protects the bacterial genome against alkylation damage. AlkB has a wide variety of substrates, including monoalkyl and exocyclic bridged adducts. Nine mammalian AlkB homologs exist (ALKBH1-8, FTO), but only a subset functions as DNA/RNA repair enzymes. This minireview presents an overview of the AlkB proteins including recent data on homologs, structural features, substrate specificities, and experimental strategies for studying DNA repair by AlkB family proteins.

引用

页码：20734 / 20742

页数：9

共 95 条

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