Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine

被引：0

作者：

Rocha, P. H. ^{[5
]}

Assis, R. A. ^{[4
]}

Lobato, F. C. F. ^{[3
]}

Cardoso, V. N. ^{[2
]}

Heneine, L. G. D. ^{[1
]}

机构：

[1] Fundacao Ezequiel Dias, BR-30510010 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Fac Farm, Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Escola Vet, Belo Horizonte, MG, Brazil

[4] MAPA, Lanagro MG, Pedro Leopoldo, MG, Brazil

[5] Univ Fed Minas Gerais, Aluno Posgrad FF, Belo Horizonte, MG, Brazil

来源：

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA | 2008年 / 60卷 / 04期

关键词：

Clostridium perfringens; epsilon toxin; iodination; stability;

D O I：

10.1590/S0102-09352008000400007

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of stability of samples kept under different temperatures 4 degrees C, - 20 degrees C, and - 80 degrees C. The iodinated prototoxins were stocked for a period of four months.

引用

页码：821 / 824

页数：4

共 50 条

[21] Antibody response in goats vaccinated with liposome-adjuvanted Clostridium perfringens type D epsilon toxoid
Uzal, FA
Wong, JP
Kelly, WR
Priest, J
VETERINARY RESEARCH COMMUNICATIONS, 1999, 23 (03) : 143 - 150
[22] The Early Effects of Clostridium perfringens Type D Epsilon Toxin in Ligated Intestinal Loops of Goats and Sheep
M.E. Fernandez Miyakawa
F.A. Uzal
Veterinary Research Communications, 2003, 27 : 231 - 241
[23] Selection of a Clostridium perfringens type D epsilon toxin producer via dot-blot test
Goncalves, Luciana A.
Lobato, Zelia I. P.
Silva, Rodrigo O. S.
Salvarani, Felipe M.
Pires, Prhiscylla S.
Assis, Ronnie A.
Lobato, Francisco C. F.
ARCHIVES OF MICROBIOLOGY, 2009, 191 (11) : 847 - 851
[24] Antibody Response in Goats Vaccinated with Liposome-adjuvanted Clostridium perfringens Type D Epsilon Toxoid
F.A. Uzal
J.P. Wong
W.R. Kelly
J. Priest
Veterinary Research Communications, 1999, 23 : 143 - 150
[25] An homogeneous fluorescence polarization assay for detection of Clostridium perfringens type D epsilon antitoxin in serum of goats
Uzal, FA
Smith, P
Nielsen, K
Lin, M
Kelly, WR
ARCHIVOS DE MEDICINA VETERINARIA, 1999, 31 (01) : 131 - 134
[26] COMPARISON OF THE NUCLEOTIDE-SEQUENCE AND DEVELOPMENT OF A PCR TEST FOR THE EPSILON TOXIN GENE OF CLOSTRIDIUM-PERFRINGENS TYPE-B AND TYPE-D
HAVARD, HL
HUNTER, SEC
TITBALL, RW
FEMS MICROBIOLOGY LETTERS, 1992, 97 (1-2) : 77 - 81
[27] In vitro effects of Clostridium perfringens type D epsilon toxin on water and ion transport in ovine and caprine intestine
Miyakawa, MEF
Ibarra, CA
Uzal, FA
ANAEROBE, 2003, 9 (03) : 145 - 149
[28] Functional and structural characterization of soluble recombinant epsilon toxin of Clostridium perfringens D, causative agent of enterotoxaemia
Mathur, Deepika Dayal
Deshmukh, Sachin
Kaushik, Himani
Garg, Lalit C.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 88 (04) : 877 - 884
[29] Outbreak of Clostridium perfringens type D enterotoxemia in sheep
Salvarani, Felipe Masiero
Faccin, Mayane
de Queiroz Ramos Freitas, Nayra Fernanda
de Matos, Monica Regina
Garcia, Edismair Carvalho
Pagliosa, Geane Maciel
Barbosa, Jose Diomedes
Faria Lobato, Francisco Carlos
Viott, Aline de Marco
SEMINA-CIENCIAS AGRARIAS, 2019, 40 (06): : 2593 - 2602
[30] Immunization with recombinant fusion of LTB and linear epitope (40-62) of epsilon toxin elicits protective immune response against the epsilon toxin of Clostridium perfringens type D
Kaushik, Himani
Deshmukh, Sachin Kumar
Solanki, Amit Kumar
Bhatia, Bharti
Tiwari, Archana
Garg, Lalit C.
AMB EXPRESS, 2019, 9 (1)

← 1 2 3 4 5 →