Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine

被引：0
作者：
Rocha, P. H. [5 ]
Assis, R. A. [4 ]
Lobato, F. C. F. [3 ]
Cardoso, V. N. [2 ]
Heneine, L. G. D. [1 ]
机构：
[1] Fundacao Ezequiel Dias, BR-30510010 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Fac Farm, Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Escola Vet, Belo Horizonte, MG, Brazil
[4] MAPA, Lanagro MG, Pedro Leopoldo, MG, Brazil
[5] Univ Fed Minas Gerais, Aluno Posgrad FF, Belo Horizonte, MG, Brazil
来源：
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA | 2008年 / 60卷 / 04期
关键词：
Clostridium perfringens; epsilon toxin; iodination; stability;
D O I：
10.1590/S0102-09352008000400007
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of stability of samples kept under different temperatures 4 degrees C, - 20 degrees C, and - 80 degrees C. The iodinated prototoxins were stocked for a period of four months.
引用
收藏
页码：821 / 824
页数：4
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