Enhanced Expression of Hedgehog Pathway Proteins in Oral Epithelial Dysplasia

被引:3
作者
Dias, Rosane Borges [1 ]
Valverde, Ludmila de Faro [1 ]
Schlaepfer Sales, Caroline Brandi [1 ]
Nazare Guimaraes, Vanessa Sousa [1 ]
Cabral, Marcia Grillo [3 ]
de Aquino Xavier, Flavia Calo [2 ]
dos Santos, Jean Nunes [2 ]
Goncalves Ramos, Eduardo Antonio [1 ]
Gurgel Rocha, Clarissa Araujo [1 ,2 ]
机构
[1] Fundacao Oswaldo Cruz, Lab Pathol & Mol Biol, Salvador, BA, Brazil
[2] Univ Fed Bahia, Sch Dent, Lab Oral Surg Pathol, BR-41170290 Salvador, BA, Brazil
[3] Univ Fed Rio de Janeiro, Sch Dent, Dept Pathol & Oral Diag, Rio De Janeiro, Brazil
关键词
oral epithelial dysplasia; Hedgehog proteins; premalignant lesion; SQUAMOUS-CELL CARCINOMA; SIGNALING PATHWAY; CYCLIN D1; CANCER; GENE; ACTIVATION; BREAST; INVOLVEMENT; MECHANISMS; NETWORK;
D O I
10.1097/PAI.0000000000000225
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The aim of this study was to characterize the profile of the proteins involved in the Hedgehog signaling pathway to aid in the understanding of the pathogenesis of oral epithelial dysplasia (OED). The proteins SHH, PTCH1, HHIP, SUFU, GLI1, and cyclin D1 were evaluated by immunohistochemistry in 25 cases of OED, 4 of non-neoplasic oral mucosa, 8 of inflammatory fibrous hyperplasia and 5 of hyperkeratosis. SHH proteins were predominant in OED cases. Although PTCH1 protein was observed in all cases, this molecule was more highly expressed in OED. The inhibitor protein SUFU was present in OED and HHIP protein was overexpressed in OED. GLI1 proteins were predominantly found in the nuclei of epithelial cells in OED. Basal and suprabasal cells in the epithelial lining were positive for cyclin D1 only in OED. In conclusion, comparative analysis of the proteins involved in the Hedgehog pathway suggests that enhanced expression of these proteins can play an important role in the biological behavior of OED.
引用
收藏
页码:595 / 602
页数:8
相关论文
共 40 条
[1]   Topical sonic hedgehog gene therapy accelerates wound healing in diabetes by enhancing endothelial progenitor cell-mediated microvascular remodeling [J].
Asai, Jun ;
Takenaka, Hideya ;
Kusano, Kengo F. ;
Ii, Masaaki ;
Luedemann, Corinne ;
Curry, Cynthia ;
Eaton, Elizabeth ;
Iwakura, Atsushi ;
Tsutsumi, Yoshiaki ;
Hamada, Hiromichi ;
Kishimoto, Saburo ;
Thorne, Tina ;
Kishore, Raj ;
Losordo, Douglas W. .
CIRCULATION, 2006, 113 (20) :2413-2424
[2]   Widespread requirement for Hedgehog ligand stimulation in growth of digestive tract tumours [J].
Berman, DM ;
Karhadkar, SS ;
Maitra, A ;
de Oca, RM ;
Gerstenblith, MR ;
Briggs, K ;
Parker, AR ;
Shimada, Y ;
Eshleman, JR ;
Watkins, DN ;
Beachy, PA .
NATURE, 2003, 425 (6960) :846-851
[3]   Gli1 Mediates Lung Cancer Cell Proliferation and Sonic Hedgehog-Dependent Mesenchymal Cell Activation [J].
Bermudez, Olga ;
Hennen, Elisabeth ;
Koch, Ina ;
Lindner, Michael ;
Eickelberg, Oliver .
PLOS ONE, 2013, 8 (05)
[4]   Activation of sonic hedgehog signaling in oral squamous cell carcinomas: a preliminary study [J].
Cavicchioli Buim, Marcilei Eliza ;
Gurgel, Clarissa Araujo S. ;
Goncalves Ramos, Eduardo Antonio ;
Lourenco, Silvia Vanessa ;
Soares, Fernando Augusto .
HUMAN PATHOLOGY, 2011, 42 (10) :1484-1490
[5]   The sonic hedgehog signaling network in development and neoplasia [J].
Chari, Nikhil S. ;
McDonnell, Timothy J. .
ADVANCES IN ANATOMIC PATHOLOGY, 2007, 14 (05) :344-352
[6]  
Che L, 2012, CHINESE J CANCER RES, V24, P323, DOI [10.3978/j.issn.1000-9604.2012.10.10, 10.1007/s11670-012-0271-z]
[7]  
Gale N., 2005, WHO CLASSIFICATION T, P140
[8]   Evidence for the involvement of the Gli gene family in embryonic mouse lung development [J].
Grindley, JC ;
Bellusci, S ;
Perkins, D ;
Hogan, BLM .
DEVELOPMENTAL BIOLOGY, 1997, 188 (02) :337-348
[9]  
Hardcastle Z, 1998, DEVELOPMENT, V125, P2803
[10]   Molecular Pathways: The Role of Primary Cilia in Cancer Progression and Therapeutics with a Focus on Hedgehog Signaling [J].
Hassounah, Nadia B. ;
Bunch, Thomas A. ;
McDermott, Kimberly M. .
CLINICAL CANCER RESEARCH, 2012, 18 (09) :2429-2435