Downregulation of EIF5A2 by miR-221-3p inhibits cell proliferation, promotes cell cycle arrest and apoptosis in medulloblastoma cells

被引:31
作者
Yang, Yong [1 ]
Cui, Hanjiang [2 ]
Wang, Xian [1 ]
机构
[1] Yangtze Univ, Affiliated Hosp 1, Dept Neurosurg, Jingzhou, Hubei, Peoples R China
[2] Second Peoples Hosp Jingzhou, Dept Neurosurg, Jingzhou, Hubei, Peoples R China
关键词
Medulloblastoma; miR-221-3p; EIF5A2; cell cycle; apoptosis; ONCOGENIC ROLE; CANCER; OVEREXPRESSION; AGGRESSIVENESS; IDENTIFICATION; EXPRESSION; CARCINOMA; REGION; RNA;
D O I
10.1080/09168451.2018.1553604
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recently, miR-221-3p expression has been reported to be down-regulated in medulloblastoma (MB), but its functional effects remains unclear. In this study, quantitative real-time PCR (qRT-PCR) revealed significantly decreased miR-221-3p in MB cell lines. Transfection of miR-221-3p mimics reduced, or inhibitor increased cell proliferation in MB cells using MTT assay. Flow cytometry analysis indicated miR-221-3p overexpression promoted, while knockdown alleviated G0/G1 arrest and apoptosis. Luciferase reporter assay confirmed miR-221-3p directly targets the EIF5A2 gene. Moreover, restoration of EIF5A2 in the miR-221-3p-overexpressing DAOY cells significantly alleviated the suppressive effects of miR-221-3p on cell proliferation, cell cycle and apoptosis. Furthermore, miR-221-3p overexpression decreased CDK4, Cyclin D1 and Bcl-2 and increased Bad expression, which was reversed by EIF5A2 overexpression. These results uncovered the tumor suppressive role of miR-221-3p in MB cell proliferation at least in part via targeting EIF5A2, suggesting that miR-221-3p might be a potential candidate target for diagnosis and therapeutics of MB.
引用
收藏
页码:400 / 408
页数:9
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