Removal of tannin from different tannin substrates using heme peroxidases of Phanerochaete chrysosporium was studied. Complete removal of tannin components in spent tan liquor was observed after 24 h of incubation with peroxidases. Tannin in aqueous media containing tannic acid and condensed tannin substrates were removed by 65.70+/-0.97 and 52.43+/-0.83%. Chemical oxygen demand was reduced by 24.38+/-0.73, 33.22+/-0.20, and 58.94+/-0.07%, respectively, in spent tan liquor, tannic acid and condensed tannin substrates containing aqueous media.