Enhancement of secondary xylem cell proliferation by Arabidopsis cyclin D overexpression in tobacco plants

被引:9
作者
Fujii, Takeo [1 ]
Sato, Kanna [1 ]
Matsui, Noriko [1 ]
Furuichi, Takayuki [1 ]
Takenouchi, Sachi [1 ]
Nishikubo, Nobuyuki [2 ]
Suzuki, Yuzo [1 ]
Kawai, Shinya [3 ]
Demura, Taku [2 ,4 ]
Kajita, Shinya [1 ]
Katayama, Yoshihiro [5 ]
机构
[1] Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
[2] RIKEN, Plant Sci Ctr, Yokohama, Kanagawa 2300045, Japan
[3] Tokyo Univ Agr & Technol, Grad Sch Agr, Fuchu, Tokyo 1838509, Japan
[4] Nara Inst Sci & Technol, Nara 6300192, Japan
[5] Nihon Univ, Coll Bioresource Sci, Fujisawa, Kanagawa 2520880, Japan
关键词
Cell cycle; Cell division; CycD; CycB; Secondary xylem; Vascular cambium; PHASE-SPECIFIC GENES; TRANSCRIPTION FACTORS; BY-2; CELLS; PROTEIN; GROWTH; TRANSACTIVATION; EXPRESSION; REGULATORS; PROMOTERS;
D O I
10.1007/s00299-012-1271-7
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Secondary xylem is composed of daughter cells produced by the vascular cambium in the stem. Cell proliferation of the secondary xylem is the result of long-range cell division in the vascular cambium. Most xylem cells have a thickened secondary cell wall, representing a large amount of biomass storage. Therefore, regulation of cell division in the vascular cambium and differentiation into secondary xylem is important for biomass production. Cell division is regulated by cell cycle regulators. In this study, we confirm that cell cycle regulators influence cell division in the vascular cambium in tobacco. We produced transgenic tobacco that expresses Arabidopsis thaliana cyclin D2;1 (AtcycD2;1) and AtE2Fa-DPa under the control of the CaMV35S promoter. Each gene is a positive regulator of the cell cycle, and is known to influence the transition from G1 phase to S phase. AtcycD2;1-overexpressing tobacco had more secondary xylem cells when compared with control plants. In order to evaluate cell division activity in the vascular cambium, we prepared a Populus trichocarpa cycB1;1 (PtcycB1;1) promoter containing a destruction box motif for ubiquitination and a beta-glucuronidase-encoding gene (PtcycB1;1pro:GUS). In transgenic tobacco containing PtcycB1;1pro:GUS, GUS staining was specifically observed in meristem tissues, such as the root apical meristem and vascular cambium. In addition, mitosis-monitoring plants containing AtcycD2;1 had stronger GUS staining in the cambium when compared with control plants. Our results indicated that overexpression of AtcycD enhances cell division in the vascular cambium and increases secondary xylem differentiation in tobacco. Key message We succeeded in inducing cell proliferation of cambium and enlargement of secondary xylem region by AtcycD overexpression. We also evaluated mitotic activity in cambium using cyclin-GUS fusion protein from poplar.
引用
收藏
页码:1573 / 1580
页数:8
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