Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes

被引：36

作者：

Colasanti, T. ^{[1
,2
]}

Vomero, M. ^{[1
]}

Alessandri, C. ^{[3
]}

Barbati, C. ^{[1
,4
]}

Maselli, A. ^{[1
]}

Camperio, C. ^{[5
]}

Conti, F. ^{[3
]}

Tinari, A. ^{[6
]}

Carlo-Stella, C. ^{[7
,8
]}

Tuosto, L. ^{[5
]}

Benincasa, D. ^{[9
,10
]}

Valesini, G. ^{[3
]}

Malorni, W. ^{[4
,11
]}

Pierdominici, M. ^{[1
]}

Ortona, E. ^{[1
,4
]}

机构：

[1] Ist Super Sanita, Dept Cell Biol & Neurosci, I-00161 Rome, Italy

[2] San Raffaele Inst Pisana, Dept Med Sci, Rome, Italy

[3] Univ Roma La Sapienza, Dept Internal Med, Lupus Clin, Rome, Italy

[4] San Raffaele Inst Sulmona, Dept Med Sci, Laquila, Italy

[5] Univ Roma La Sapienza, Ist Pasteur, Fdn Cenci Bolognetti, Dept Biol & Biotechnol C Darwin, Rome, Italy

[6] Ist Super Sanita, Dept Technol & Hlth, I-00161 Rome, Italy

[7] Humanitas Clin & Res Ctr, Humanitas Canc Ctr, Dept Hematol & Oncol, Rozzano, MI, Italy

[8] Univ Milan, Dept Med Biotechnol & Translat Med, I-20122 Milan, Italy

[9] IRCCS, Fdn Santa Lucia, Lab Clin & Behav Neurol, Rome, Italy

[10] Univ Roma La Sapienza, SantAndrea Hosp, Movement Disorder Unit, Rome, Italy

[11] Ist Super Sanita, Dept Therapeut Res & Med Evaluat, I-00161 Rome, Italy

来源：

CELL DEATH & DISEASE | 2014年 / 5卷

关键词：

alpha-synuclein; T lymphocytes; autophagy; systemic lupus erythematosus; lymphoma; DISEASE-ACTIVITY; APOPTOSIS; IMMUNITY; CELL; MACROAUTOPHAGY; AGGREGATION; EXPRESSION; SORAFENIB; PATHWAYS; SURVIVAL;

D O I：

10.1038/cddis.2014.211

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

It has been demonstrated that alpha-synuclein can aggregate and contribute to the pathogenesis of some neurodegenerative diseases and it is capable of hindering autophagy in neuronal cells. Here, we investigated the implication of alpha-synuclein in the autophagy process in primary human T lymphocytes. We provide evidence that: (i) knocking down of the alpha-synuclein gene resulted in increased autophagy, (ii) autophagy induction by energy deprivation was associated with a significant decrease of a-synuclein levels, (iii) autophagy inhibition by 3-methyladenine or by ATG5 knocking down led to a significant increase of alpha-synuclein levels, and (iv) autophagy impairment, constitutive in T lymphocytes from patients with systemic lupus erythematosus, was associated with abnormal accumulation of Phi-synuclein aggregates. These results suggest that alpha-synuclein could be considered as an autophagy-related marker of peripheral blood lymphocytes, potentially suitable for use in the clinical practice.

引用

页码：e1265 / e1265

页数：9

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