Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo

被引：37
作者：
Wang, Xueting [1 ,2 ,3 ]
Chen, Yu [1 ,2 ,3 ]
Zuo, Xintong [4 ]
Ding, Naiqing [1 ]
Zeng, Huijuan [1 ]
Zou, Xiang [5 ]
Han, Xiaodong [1 ,2 ,3 ]
机构：
[1] Nanjing Univ, Sch Med, Immunol & Reprod Biol Lab, Nanjing 210093, Jiangsu, Peoples R China
[2] Nanjing Univ, Jiangsu Key Lab Mol Med, Nanjing 210093, Jiangsu, Peoples R China
[3] Nanjing Univ, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China
[4] Mt Holyoke Coll, Dept Biochem, S Hadley, MA 01075 USA
[5] Univ Gothenburg, Inst Biomed, Dept Microbiol & Immunol, Mucosal Immunobiol & Vaccine Res Ctr, Gothenburg, Sweden
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2013年 / 60卷
基金：
中国国家自然科学基金;
关键词：
Microcystin-LR; Apoptosis; Testicular cells; Mouse; DNA-DAMAGE; PROTEIN; P53; EXPRESSION; TOXIN; LAKE; BAX; INHIBITION; AERUGINOSA; NODULARIN;
D O I：
10.1016/j.fct.2013.07.039
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Microcystin-LR (MC-LR) can induce apoptosis of a wide range of tissue cells including testicular cells. The purpose of the study was to find out whether the expression and phosphorylation of p53, Bcl-2 protein family proteins, Cyt c, and caspases were involved in the induction of testicular cell apoptosis by MC-LR in mice. Results showed that following exposure to MC-LR, expression of Bax, caspase 3 and caspase 8 was up-regulated. Significant increases in the phosphorylation of both p53 and Bcl-2 were identified after the administration of MC-LR. The administration of MC-LR also resulted in significant increases of c-myc, c-jun, and c-fos. In conclusion, p53, Bcl-2, Bax, Caspase 3 and Caspase 8 are involved in the regulation of MC-LR-induced apoptosis of testicular cells. The overexpression of c-myc, c-jun and c-fos suggests that MC-LR may have carcinogenic potential for testes. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：309 / 317
页数：9
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