Characterization of Pythium Species Collected from a Multiple Time-Point Sampling of Cucurbits in South Carolina

被引:7
|
作者
Toporek, Sean M. [1 ]
Keinath, Anthony P. [1 ]
机构
[1] Clemson Univ, Coastal Res & Educ Ctr, Dept Plant & Environm Sci, Charleston, SC 29414 USA
基金
美国食品与农业研究所;
关键词
Pythium; cucurbits; survey; COX1; oxathiapiprolin; DAMPING-OFF; MOLECULAR PHYLOGENY; FLORICULTURE CROPS; SOYBEAN SEEDLINGS; ROOT-ROT; SOIL; SENSITIVITY; DIVERSITY; SPP; OXATHIAPIPROLIN;
D O I
10.1094/PDIS-04-20-0728-RE
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Species of Pythium cause root and stem rot in cucurbits, but no formal surveys have been conducted in the United States to identify which species are responsible. The cucurbit hosts bottle gourd, cucumber, Hubbard squash, and watermelon were transplanted in May, July, September, and November into sentinel plots in four and five different fields in 2017 and 2018, respectively, in South Carolina. Eight of the nine fields were replanted in March 2019. Isolates (600) were collected and identified by sequencing DNA of the mitochondrial cytochrome oxidase I region. The four most common species were P. spinosum (45.6% of all isolates), P. myriotylum (20.0%), P. irregulare (15.3%), and P. aphanidermatum (12.8%). P. myriotylum and P. aphanidermatum were predominantly isolated in May, July, and September, whereas P. spinosum and P. irregulare were predominantly isolated in November and March. Isolates of P. ultimum, P. irregulare, and P. spinosum were more virulent than isolates of P. myriotylum and P. aphanidermatum at 25 degrees C. Representative isolates were screened in vitro for sensitivity to three fungicides: mefenoxam, propamocarb, and oxathiapiprolin. All isolates were sensitive to mefenoxam and propamocarb, but these same isolates were insensitive to oxathiapiprolin, except those classified taxonomically in Pythium Glade I.
引用
收藏
页码:2832 / 2842
页数:11
相关论文
共 12 条
  • [1] Time-point field sampling of Pythium spp. pathogenic on cucurbits reveals a seasonal change in species recovery
    Toporek, S.
    Keinath, A. P.
    PHYTOPATHOLOGY, 2019, 109 (10) : 171 - 171
  • [2] A COMBINED STUDY OF CORTISOL, ACTH AND DEXAMETHASONE CONCENTRATIONS IN MAJOR DEPRESSION - MULTIPLE TIME-POINT SAMPLING
    CHARLTON, BG
    LEAKE, A
    WRIGHT, C
    GRIFFITHS, HW
    FERRIER, IN
    BRITISH JOURNAL OF PSYCHIATRY, 1987, 150 : 791 - 796
  • [3] Identification and characterization of Armillaria species from South Carolina
    Schnabel, G.
    Ash, J. S.
    Simpson, C. R.
    Bryson, K. K.
    PHYTOPATHOLOGY, 2004, 94 (06) : S93 - S93
  • [4] A new species of Pythium producing multiple oospores isolated from bentgrass in North Carolina
    Abad, G
    Shew, HD
    Grand, LF
    Lucas, LT
    MYCOLOGIA, 1995, 87 (06) : 896 - 901
  • [5] Characterization of Cytospora species from South Carolina Peach Trees
    Boatwright, H.
    Schnabel, G.
    PHYTOPATHOLOGY, 2019, 109 (10) : 122 - 122
  • [6] Identification and characterization of Pythium species isolated from commercial alfalfa fields in South Dakota
    Rebstock, T.
    Rachynska, O.
    Giles, J.
    Tordsen, C.
    Sathoff, A. E.
    PHYTOPATHOLOGY, 2022, 112 (11) : 114 - 115
  • [7] CHARACTERIZATION AND PATHOGENICITY OF PYTHIUM SPECIES ISOLATED FROM TURFGRASS WITH SYMPTOMS OF ROOT AND CROWN ROT IN NORTH-CAROLINA
    ABAD, ZG
    SHEW, HD
    LUCAS, LT
    PHYTOPATHOLOGY, 1994, 84 (09) : 913 - 921
  • [8] Multiple time-point fitting: Improving absolute measurements of myocardial wall thickening from gated perfusion/metabolism studies.
    Mansour, P
    Bartlett, ML
    Bacharach, SL
    JOURNAL OF NUCLEAR MEDICINE, 1997, 38 (05) : 474 - 474
  • [9] "ACTIVE" PASSIVE SAMPLING IN TWO SPECIES OF LEPOMIS FROM PAR POND, SOUTH CAROLINA, UNITED STATES: A CASE STUDY OF INFRACOMMUNITY NESTEDNESS
    Zelmer, Derek A.
    Gross, Christine M.
    JOURNAL OF PARASITOLOGY, 2009, 95 (05) : 1054 - 1061
  • [10] Multiple Time-Point 68Ga-PSMA I&T PET/CT for Characterization of Primary Prostate Cancer: Value of Early Dynamic and Delayed Imaging
    Schmuck, Sebastian
    Mamach, Martin
    Wilke, Florian
    von Klot, Christoph A.
    Henkenberens, Christoph
    Thackeray, James T.
    Sohns, Jan M.
    Geworski, Lilli
    Ross, Tobias L.
    Wester, Hans-Juergen
    Christiansen, Hans
    Bengel, Frank M.
    Derlin, Thorsten
    CLINICAL NUCLEAR MEDICINE, 2017, 42 (06) : e286 - e293