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Characterization of Pythium Species Collected from a Multiple Time-Point Sampling of Cucurbits in South Carolina
被引:7
|作者:
Toporek, Sean M.
[1
]
Keinath, Anthony P.
[1
]
机构:
[1] Clemson Univ, Coastal Res & Educ Ctr, Dept Plant & Environm Sci, Charleston, SC 29414 USA
基金:
美国食品与农业研究所;
关键词:
Pythium;
cucurbits;
survey;
COX1;
oxathiapiprolin;
DAMPING-OFF;
MOLECULAR PHYLOGENY;
FLORICULTURE CROPS;
SOYBEAN SEEDLINGS;
ROOT-ROT;
SOIL;
SENSITIVITY;
DIVERSITY;
SPP;
OXATHIAPIPROLIN;
D O I:
10.1094/PDIS-04-20-0728-RE
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
Species of Pythium cause root and stem rot in cucurbits, but no formal surveys have been conducted in the United States to identify which species are responsible. The cucurbit hosts bottle gourd, cucumber, Hubbard squash, and watermelon were transplanted in May, July, September, and November into sentinel plots in four and five different fields in 2017 and 2018, respectively, in South Carolina. Eight of the nine fields were replanted in March 2019. Isolates (600) were collected and identified by sequencing DNA of the mitochondrial cytochrome oxidase I region. The four most common species were P. spinosum (45.6% of all isolates), P. myriotylum (20.0%), P. irregulare (15.3%), and P. aphanidermatum (12.8%). P. myriotylum and P. aphanidermatum were predominantly isolated in May, July, and September, whereas P. spinosum and P. irregulare were predominantly isolated in November and March. Isolates of P. ultimum, P. irregulare, and P. spinosum were more virulent than isolates of P. myriotylum and P. aphanidermatum at 25 degrees C. Representative isolates were screened in vitro for sensitivity to three fungicides: mefenoxam, propamocarb, and oxathiapiprolin. All isolates were sensitive to mefenoxam and propamocarb, but these same isolates were insensitive to oxathiapiprolin, except those classified taxonomically in Pythium Glade I.
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页码:2832 / 2842
页数:11
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