Effect of NeuroD2 expression on neuronal differentiation in mouse embryonic stem cells

被引：13

作者：

Sugimoto, Yasutaka ^{[2
]}

Furuno, Tadahide ^{[1
,2
]}

Nakanishi, Mamoru ^{[1
,2
]}

机构：

[1] Aichi Gakuin Univ, Sch Pharm, Chikusa Ku, Nagoya, Aichi 4648650, Japan

[2] Nagoya City Univ, Grad Sch Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi 4678603, Japan

来源：

CELL BIOLOGY INTERNATIONAL | 2009年 / 33卷 / 02期

关键词：

NeuroD2; Embryonic stem cells; Doxycycline; Neuron; LOOP-HELIX PROTEIN; MOLECULAR-CLONING; MAMMALIAN-CELLS; GENE-EXPRESSION; IN-VITRO;

D O I：

10.1016/j.cellbi.2008.10.010

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A basic helix-loop-helix transcriptional factor, NeuroD2, plays important roles in neuronal differentiation and survival. We introduced the tetracycline-dependent NeuroD2 expression system to embryonic stem (ES) cells and studied the role of NeuroD2 in the neuronal differentiation. The addition of doxycycline induced the expression of NeuroD2 after 24 h and the differentiation to neurons after 3 days in ES cells, which are transfected with vectors composed of reverse tetracycline-controlled transactivator with cytomegarovirus promoter and NeuroD2 with tetracycline response element. Treatment with doxycycline for 3 days induced neuronal differentiation, but not within 1 day; furthermore NeuroD2 was detected in the nucleus 3 days after treatment, but also not within 1 day. The results suggest that the expression of NeuroD2 requires an appropriate period of about 3 days to elicit neuronal differentiation in ES cells. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：174 / 179

页数：6

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