Effect of four materials including trehalose, soluble starch, raffinose and galactose on survival of Lactobacillus acidophilus was studied by counting viable cells before and after freeze-drying. The concentrations of five carbohydrates were all 3%, 6%, 9%, 12% and 15%. Results showed as follows: the optimal concentrations of trehalose, soluble starch, raffinose and galactose were 12%, 9%, 3% and 15%, the survival of Lactobacillus acidophilus was 42.62%, 56.50%, 45.05% and 42.69% and the viable cells of lyophilized powder were 0.40x10(11)cfu/g,0.45x10(11)cfu/g, 0.52x10(11) and 1.09x10(11)cfu/g, respectively.