Evidence for a mediator cycle at the initiation of transcription

被引：102

作者：

Svejstrup, JQ ^{[1
]}

Li, Y ^{[1
]}

Fellows, J ^{[1
]}

Gnatt, A ^{[1
]}

Bjorklund, S ^{[1
]}

Kornberg, RD ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,CLARE HALL LABS,S MIMMS EN6 3LD,HERTS,ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 12期

关键词：

RNA polymerase II; C-terminal domain; yeast;

D O I：

10.1073/pnas.94.12.6075

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Free and elongating (DNA-bound) forms of RNA polymerase II were separated from yeast. Most cellular polymerase II was found in the elongating fraction, which contained all enzyme phosphorylated on the C-terminal domain and none of the 15-subunit mediator of transcriptional regulation, These and other findings suggest that mediator enters and leaves initiation complexes during every round of transcription, in a process that may be coupled to C-terminal domain phosphorylation.

引用

页码：6075 / 6078

页数：4

共 19 条

[1] A minimal set of RNA polymerase II transcription protein interactions [J].

Bushnell, DA ;

Bamdad, C ;

Kornberg, RD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (33) :20170-20174

[2] A MULTISUBUNIT COMPLEX CONTAINING THE SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, AND SNF6 GENE-PRODUCTS ISOLATED FROM YEAST [J].

CAIRNS, BR ;

KIM, YJ ;

SAYRE, MH ;

LAURENT, BC ;

KORNBERG, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (05) :1950-1954

[3] GAL4 PROTEIN - PURIFICATION, ASSOCIATION WITH GAL80 PROTEIN, AND CONSERVED DOMAIN-STRUCTURE [J].

CHASMAN, DI ;

KORNBERG, RD .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (06) :2916-2923

[4] THE ROLE OF MULTISITE PHOSPHORYLATION IN THE REGULATION OF RNA-POLYMERASE-II ACTIVITY [J].

DAHMUS, ME .

PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 48, 1994, 48 :143-179

[5] CTD KINASE ASSOCIATED WITH YEAST RNA POLYMERASE-II INITIATION FACTOR-B [J].

FEAVER, WJ ;

GILEADI, O ;

LI, Y ;

KORNBERG, RD .

CELL, 1991, 67 (06) :1223-1230

[6]

FLANAGAN PM, 1990, J BIOL CHEM, V265, P11105

[7] A MEDIATOR REQUIRED FOR ACTIVATION OF RNA POLYMERASE-II TRANSCRIPTION INVITRO [J].

FLANAGAN, PM ;

KELLEHER, RJ ;

SAYRE, MH ;

TSCHOCHNER, H ;

KORNBERG, RD .

NATURE, 1991, 350 (6317) :436-438

[8]

GUSTAFSSON CM, 1996, J BIOL CHEM, V272, P48

[9] A NOVEL MEDIATOR BETWEEN ACTIVATOR PROTEINS AND THE RNA POLYMERASE-II TRANSCRIPTION APPARATUS [J].

KELLEHER, RJ ;

FLANAGAN, PM ;

KORNBERG, RD .

CELL, 1990, 61 (07) :1209-1215

[10] A MULTIPROTEIN MEDIATOR OF TRANSCRIPTIONAL ACTIVATION AND ITS INTERACTION WITH THE C-TERMINAL REPEAT DOMAIN OF RNA-POLYMERASE-II [J].

KIM, YJ ;

BJORKLUND, S ;

LI, Y ;

SAYRE, MH ;

KORNBERG, RD .

CELL, 1994, 77 (04) :599-608

← 1 2 →