Novel wound dressing based on nanofibrous PHBV-keratin mats

被引：58

作者：

Yuan, Jiang ^{[1
,2
]}

Geng, Jia ^{[1
]}

Xing, Zhicai ^{[1
]}

Shim, Kyoung-Jin ^{[3
]}

Han, Insook ^{[3
]}

Kim, Jung-Chul ^{[3
]}

Kang, Inn-Kyu ^{[1
]}

Shen, Jian ^{[2
]}

机构：

[1] Kyungpook Natl Univ, Dept Polymer Sci, Taegu 702701, South Korea

[2] Nanjing Normal Univ, Coll Chem & Mat Sci, Jiangsu Key Lab Biofunct Mat, Nanjing, Jiangsu, Peoples R China

[3] Kyungpook Natl Univ, Sch Med, Dept Immunol, Taegu 702701, South Korea

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2015年 / 9卷 / 09期

基金：

中国国家自然科学基金;

关键词：

wound dressing; keratin; PHBV; electrospinning; nanofibre; CARBOXYMETHYLATED FEATHER KERATINS; HUMAN HAIR; CROSS-LINKING; SILK FIBROIN; BIOMATERIAL; FABRICATION; SCAFFOLDS; SKIN; PROLIFERATION; FIBROBLASTS;

D O I：

10.1002/term.1653

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Keratin is an important protein used for wound healing and tissue recovery. In this study, keratin was first extracted from raw materials and chemically modified to obtain stable keratin (m-keratin). The raw and m-keratin were examined by Raman spectroscopy. The molecular weight of the m-keratin was analysed by SDS-PAGE. The m-keratin was then blended with poly(hydroxybutylate-co-hydroxyvalerate) (PHBV) and electrospun to afford nanofibrous mats. These mats were characterized by field emission scanning electron microscopy (FE-SEM), electron spectroscopy for chemical analysis (ESCA) and atomic forcemicroscopy (AFM). Fromthe 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) data, it was found that introduction of keratin enhanced cell proliferation. From wound-healing test and histological examination results, it was shown that the composite mats accelerated wound recovery remarkably as compared to the PHBV control. It was concluded that PHBV-keratin may be a good candidate as a wound dressing. Copyright (C) 2012 John Wiley & Sons, Ltd.

引用

页码：1027 / 1035

页数：9

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