The adsorption mechanism of titanium-binding ferritin to amphoteric oxide

被引：9

作者：

Fukuta, Megumi ^{[1
,3
]}

Zettsu, Nobuyuki ^{[1
,3
]}

Yamashita, Ichiro ^{[2
,3
]}

Uraoka, Yukiharu ^{[2
,3
]}

Watanabe, Heiji ^{[1
,3
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Nara Inst Sci & Technol, Grad Sch Mat Sci, Nara 6300101, Japan

[3] Japan Sci & Technol Agcy, CREST, Chiyoda Ku, Tokyo 1020075, Japan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2013年 / 102卷

基金：

日本科学技术振兴机构;

关键词：

Ferritin; Titanium-binding peptide; Adsorption mechanism; Selective adsorption; Amphoteric oxide; PROTEIN CAGE; BIOMIMETIC SYNTHESIS; NANOPARTICLES; FABRICATION; SURFACE; ARRAY; IMMOBILIZATION; SPECIFICITY; SELECTION; AFFINITY;

D O I：

10.1016/j.colsurfb.2012.07.042

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We investigated the origin of selective adsorption of titanium-binding ferritin (TBF), the outer surface of which is genetically modified with titanium-binding peptides (TBPs). By varying pH conditions (7-9), TBF adsorption behavior onto amphoteric and acidic oxide substrates was observed using atomic force microscopy, and the zeta potential of substrates was measured. This suggests that a TBP interacted with local charges such as -O-, -OH+, and -OH2+ on substrates regardless of the constituent elements of the substrate, which makes it possible for TBF to adsorb on TiOX, ZrO2, Fe2O3, and SiO2 substrates despite the presence of an overall electrostatic repulsive force between TBF and the substrates. This also suggests that a surfactant, TWEEN20, can completely hamper attractive interaction between TBF and acidic oxide, but amphoteric oxide can withstand TWEEN20 interference. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：435 / 440

页数：6

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