A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis

被引：10

作者：

Shah, Jyotsna S. ^{[1
,2
]}

Mark, Olivia ^{[2
]}

Caoili, Eddie ^{[2
]}

Poruri, Akhila ^{[2
]}

Horowitz, Richard I. ^{[3
,4
]}

Ashbaugh, Alan D. ^{[5
]}

Ramasamy, Ranjan ^{[1
]}

机构：

[1] ID FISH Technol Inc, 556 Gibraltar Dr, Milpitas, CA 95035 USA

[2] IGenex Inc, 556 Gibraltar Dr, Milpitas, CA 95035 USA

[3] Hudson Valley Healing Arts Ctr, New York, NY 12538 USA

[4] US Dept Hlth & Human Serv, HHS Subcomm Babesia & Tick Borne Pathogens, Washington, DC 20201 USA

[5] Univ Cincinnati, Coll Med, Cincinnati, OH 45221 USA

来源：

DIAGNOSTICS | 2020年 / 10卷 / 06期

关键词：

Babesia; babesiosis; FISH; fluorescence in situ hybridization; laboratory diagnosis of babesiosis; INFECTION; PATHOGENS; ASSAY;

D O I：

10.3390/diagnostics10060377

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Apicomplexan parasites of the genusBabesiacause babesiosis in humans and animals. The microscopic examination of stained blood smears, detection of serum antibodies by immunoassays, and PCR-based identification of parasite nucleic acid in blood are common laboratory methods for diagnosing babesiosis. The present study evaluated a commercially availableBabesiagenus-specific fluorescence in situ hybridization (FISH) test for detectingBabesiaparasites in blood smears. The FISH test detectedBabesia duncaniandBabesia microti, two common species that cause human infections in the USA, and otherBabesiaspecies of human and veterinary importance in less than two hours. TheBabesiagenus-specific FISH test supplements other existing laboratory methods for diagnosing babesiosis and may be particularly useful in resource-limited laboratories.

引用

页数：9

共 31 条

[1] A novel quantitative PCR detects Babesia infection in patients not identified by currently available non-nucleic acid amplification tests [J].

Akoolo, Lavoisier ;

Schlachter, Samantha ;

Khan, Rasel ;

Alter, Laura ;

Rojtman, Albert D. ;

Gedroic, Kristine ;

Bhanot, Purnima ;

Parveen, Nikhat .

BMC MICROBIOLOGY, 2017, 17

[2] Transmission of Cytauxzoon felis to domestic cats by Amblyomma americanum nymphs [J].

Allen, Kelly E. ;

Thomas, Jennifer E. ;

Wohltjen, Megan L. ;

Reichard, Mason V. .

PARASITES & VECTORS, 2019, 12 (1)

[3] Diagnostic Tools for the Identification of Babesia sp. in Persistently Infected Cattle [J].

Alvarez, J. Antonio ;

Rojas, Carmen ;

Figueroa, Julio V. .

PATHOGENS, 2019, 8 (03)

[4] Rapid method for detecting and differentiating Mycobacterium tuberculosis complex and non-tuberculous mycobacteria in sputum by fluorescence in situ hybridization with DNA probes [J].

Baliga, Shrikala ;

Murphy, Christina ;

Sharon, Leesha ;

Shenoy, Suchitra ;

Biranthabail, Dhanashree ;

Weltman, Helena ;

Miller, Steve ;

Ramasamy, Ranjan ;

Shah, Jyotsna .

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2018, 75 :1-7

[5] Transmission of Babesia microti Parasites by Solid Organ Transplantation [J].

Brennan, Meghan B. ;

Herwaldt, Barbara L. ;

Kazmierczak, James J. ;

Weiss, John W. ;

Klein, Christina L. ;

Leith, Catherine P. ;

He, Rong ;

Oberley, Matthew J. ;

Tonnetti, Laura ;

Wilkins, Patricia P. ;

Gauthier, Gregory M. .

EMERGING INFECTIOUS DISEASES, 2016, 22 (11) :1869-1876

[6]

Centers for Disease Control and Prevention, 2015, LYM DIS

[7]

Centers for Disease Control and Prevention, 2020, PAR BAB

[8]

Centers for Disease Control and Prevention, 2015, TICK BORN REL FEV TB

[9] Description of Babesia duncani n.sp (Apicomplexa: Babesiidae) from humans and its differentiation from other piroplasms [J].

Conrad, Patricia A. ;

Kjemtrup, Anne M. ;

Carreno, Ramon A. ;

Thomford, John ;

Wainwright, Katlyn ;

Eberhard, Mark ;

Quick, Rob ;

Telford, Sam R., III ;

Herwaldt, Barbara L. .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2006, 36 (07) :779-789

[10] PHYLOGENETIC STAINS - RIBOSOMAL RNA-BASED PROBES FOR THE IDENTIFICATION OF SINGLE CELLS [J].

DELONG, EF ;

WICKHAM, GS ;

PACE, NR .

SCIENCE, 1989, 243 (4896) :1360-1363

← 1 2 3 4 →