High-Resolution Structure of a Protein Spin-Label in a Solvent-Exposed β-Sheet and Comparison with DEER Spectroscopy

被引:28
作者
Cunningham, Timothy F. [1 ]
McGoff, Marshall S. [1 ]
Sengupta, Ishita [2 ]
Jaroniec, Christopher P. [2 ]
Horne, W. Seth [1 ]
Saxena, Sunil [1 ]
机构
[1] Univ Pittsburgh, Dept Chem, Pittsburgh, PA 15260 USA
[2] Ohio State Univ, Dept Chem, Columbus, OH 43210 USA
基金
美国国家科学基金会;
关键词
SOLID-STATE NMR; IMMUNOGLOBULIN-BINDING DOMAIN; NUCLEAR-MAGNETIC-RESONANCE; NITROXIDE SIDE-CHAIN; T4; LYSOZYME; MEMBRANE-PROTEINS; CRYSTAL-STRUCTURES; EPR-SPECTRA; DYNAMICS; MOTION;
D O I
10.1021/bi300328w
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
X-ray crystallography has been a useful tool in the development of site-directed spin labeling by resolving rotamers of the nitroxide spin-label side chain in a variety of alpha-helical environments. In this work, the crystal structure of a doubly spin-labeled N8C/K28C mutant of the B1 immunoglobulin-binding domain of protein G (GB1) was solved. The double mutant formed a domain-swapped dimer under crystallization conditions. Two rotameric states of the spin-label were resolved at the solvent-exposed a-helical site, at residue 28; these are in good agreement with rotamers previously reported for helical structures. The second site, at residue 8 on an interior beta-strand, shows the presence of three distinct solvent-exposed side-chain rotamers. One of these rotamers is rarely observed within crystal structures of R1 sites and suggests that the H-alpha and S-delta hydrogen bond that is common to alpha-helical sites is absent at this interior beta-strand residue. Variable temperature continuous wave (CW) experiments of the beta-strand site showed two distinct components that were correlated to the rotameric states observed in crystallography. Interestingly, the CW data at room temperature could be fit without the use of an order parameter, which is consistent with the lack of the H-alpha and S-delta interaction. Additionally, double electron electron resonance (DEER) spectroscopy was performed on the GB1 double mutant in its monomeric form and yielded a most probable interspin distance of 25 +/- 1 angstrom. In order to evaluate the accuracy of the measured DEER distance, the rotamers observed in the crystal structure of the domain-swapped GB1 dimer were modeled into a high-resolution structure of the wild type monomeric GB1. The distances generated in the resulting GB1 structural models match the most probable DEER distance within similar to 2 angstrom. The results are interesting as they indicate by direct experimental measurement that the rotameric states of R1 found in this crystal provide a very close match to the most probable distance measured by DEER
引用
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页码:6350 / 6359
页数:10
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