Synthesis, characterization and cytotoxicity of TMC-graft-poly(vinyl alcohol) copolymers

被引：17

作者：

Martins, Alessandro F. ^{[1
]}

Bueno, Pedro V. A. ^{[1
]}

Follmann, Heveline D. M. ^{[1
,2
]}

Nocchi, Samara R. ^{[2
]}

Nakamura, Celso V. ^{[2
]}

Rubira, Adley F. ^{[1
]}

Muniz, Edvani C. ^{[1
]}

机构：

[1] Univ Estadual Maringa, Dept Quim, GMPC, BR-87020900 Maringa, Parana, Brazil

[2] Univ Estadual Maringa, Lab Inovacao Tecnol Desenvolvimento Farmacos & Co, BR-87020900 Maringa, Parana, Brazil

来源：

CARBOHYDRATE RESEARCH | 2013年 / 381卷

关键词：

Chitosan; N-trimethyl chitosan; Poly(vinyl alcohol); Copolymers; Graft; TRIMETHYL CHITOSAN CHLORIDE; POLY(VINYL ALCOHOL); DRUG-DELIVERY; N; N-TRIMETHYL CHITOSAN; BLOCK-COPOLYMERS; IN-VITRO; QUATERNIZATION; DERIVATIVES; COMPLEXES; HYDROGELS;

D O I：

10.1016/j.carres.2012.11.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-trimethyl chitosan-graft-poly(vinyl alcohol) (TMC-g-PVA) copolymers were prepared. The grafting reactions were conducted in water changing the feed ratios of poly(vinyl alcohol)/6-O-succinate-N-trimethyl chitosan (PVA/STMC). The structure of TMC-g-PVA copolymers was characterized through H-1 NMR spectroscopy, thermogravimetric analysis (TGA/DTG), wide-angle X-ray scattering (WAXS) and scanning electron microscopy (SEM). The quaternization degree (DQ) and substitution degree (DS) of N-trimethyl chitosan (TMC) and 6-O-succinate-N-trimethyl chitosan (STMC) were determined by H-1 NMR, being the spectroscopy 14.0 and 5.5 mol-% found, respectively. The viability of HCT-116 cancerous cells was investigated at different concentrations. The effect of PVA/STMC ratios on the cytotoxicity of the TMC-g-PVA copolymers was examined and the CC50 values determined for every case. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：153 / 160

页数：8

共 33 条

[1] Regioselective pivaloylation of N-phthaloylchitosan: a promising soluble intermediate for chitosan chemistry [J].

Babin, Mathieu ;

Ruest, Amelie ;

Drouin, Guillaume ;

Sirois, Kathleen ;

Ouellet, Steeven ;

Gagnon, Jonathan .

CARBOHYDRATE RESEARCH, 2012, 351 :87-92

[2]

BRITTO D, 2004, POLYM DEGRAD STABIL, V84, P353

[3]

BRITTO D, 2008, CARBOHYD POLYM, V74, P86

[4] Preparation and properties of a novel thermosensitive N-trimethyl chitosan hydrogel [J].

Chang, Yuhua ;

Xiao, Ling ;

Du, Yumin .

POLYMER BULLETIN, 2009, 63 (04) :531-545

[5] Viscosity behavior of chitosan and N,N,N-trimethylchitosan chloride salts in acid-free aqueous solution [J].

Curti, E ;

Campana-Filho, SP .

JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, 2006, A43 (03) :555-572

[6] Gene delivery using chitosan, trimethyl chitosan or polyethylenglycol-graft-trimethyl chitosan block copolymers: Establishment of structure-activity relationships in vitro [J].

Germershaus, Oliver ;

Mao, Shirui ;

Sitterberg, Johannes ;

Bakowsky, Udo ;

Kissel, Thomas .

JOURNAL OF CONTROLLED RELEASE, 2008, 125 (02) :145-154

[7] Biodegradable Poly(vinyl alcohol)-polyethylenimine Nanocomposites for Enhanced Gene Expression In Vitro and In Vivo [J].

Goyal, Ritu ;

Tripathi, Sushil K. ;

Vazquez, Esther ;

Kumar, Pradeep ;

Gupta, Kailash C. .

BIOMACROMOLECULES, 2012, 13 (01) :73-83

[8] Preparation, characterization, and properties of chitosan-g-poly(vinyl alcohol) copolymer [J].

Huang, MF ;

Fang, Y .

BIOPOLYMERS, 2006, 81 (03) :160-166

[9] Physicochemical properties and biocompatibility of N-trimethyl chitosan: Effect of quaternization and dimethylation [J].

Jintapattanakit, Anchalee ;

Mao, Shirui ;

Kissel, Thomas ;

Junyaprasert, Varaporn Buraphacheep .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2008, 70 (02) :563-571

[10] Synthesis and characterization of PEG-graft-quaternized chitosan and cationic polymeric liposomes for drug delivery [J].

Liang, Xiaofei ;

Sun, Yanming ;

Duan, Yourong ;

Cheng, Yingsheng .

JOURNAL OF APPLIED POLYMER SCIENCE, 2012, 125 (02) :1302-1309

← 1 2 3 4 →