Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula

被引：41
作者：
Qian, Lei [1 ]
Liu, Han [2 ]
Li, Tao [3 ]
Liu, Yihan [4 ]
Zhang, Zhijun [1 ]
Zhang, Yeni [2 ]
机构：
[1] Tianjin Acad Agr Sci, Natl Engn Technol Res Ctr Preservat Agr Prod, Res Inst Storage & Proc Technol Agr Prod,Tianjin, Key Lab Storage Agr Prod,Minist Agr & Rural Affai, Tianjin 300384, Peoples R China
[2] Tianjin Agr Univ, Coll Food Sci & Biotechnol, Tianjin 300384, Peoples R China
[3] Tianjin Agr Univ, Coll Basic Sci, Tianjin 300384, Peoples R China
[4] Tianjin Univ Sci & Technol, Coll Biotechnol, Key Lab Ind Fermentat Microbiol, Tianjin Key Lab Ind Microbiol,Minist Educ, Tianjin 300457, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 162卷
关键词：
Auricularia auricula; Polysaccharide; Antioxidant; ASSISTED EXTRACTION; OXIDATIVE STRESS; STRUCTURAL-CHARACTERIZATION; CHEMICAL-CHARACTERIZATION; CRUDE POLYSACCHARIDES; FRUITING BODIES; MUSHROOM; OPTIMIZATION; PROTEIN; MECHANISMS;
D O I：
10.1016/j.ijbiomac.2020.07.314
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The objective was to investigate the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula. AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography. The molecular weight and monosaccharide composition were determined by high performance gel permeation chromatography and high-performance liquid chromatography, respectively. Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization. The results showed that AAP-3-1 is a heteropolysaccharide and is mainly composed of mannose and glucose in a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa. AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively. AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase. The results indicated that the antioxidant mechanism of AAP-3-1 was associated with both non-enzymatic and enzymatic defense systems. (C) 2020 Elsevier B.V. All rights reserved.
引用
收藏
页码：1453 / 1464
页数：12
相关论文
共 63 条
[1]   Ultrasonically extracted β-D-glucan from artificially cultivated mushroom, characteristic properties and antioxidant activity [J].
Alzorqi, Ibrahim ;
Sudheer, Surya ;
Lu, Ting-Jang ;
Manickam, Sivakumar .
ULTRASONICS SONOCHEMISTRY, 2017, 35 :531-540
[2]   A review of the polysaccharide, protein and selected nutrient content of Auricularia, and their potential pharmacological value [J].
Bandara, A. R. ;
Rapior, S. ;
Mortimer, P. E. ;
Kakumyan, P. ;
Hyde, K. D. ;
Xu, J. .
MYCOSPHERE, 2019, 10 (01) :579-607
[3]   Natural Antioxidants: Sources, Compounds, Mechanisms of Action, and Potential Applications [J].
Brewer, M. S. .
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2011, 10 (04) :221-247
[4]   Extraction, Antimicrobial, and Antioxidant Activities of Crude Polysaccharides from the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Higher Basidiomycetes) [J].
Cai, Ming ;
Lin, Yang ;
Luo, Yin-long ;
Liang, Han-hua ;
Sun, Pei-long .
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS, 2015, 17 (06) :591-600
[5]   Comparison of Antioxidant and Antiproliferation Activities of Polysaccharides from Eight Species of Medicinal Mushrooms [J].
Chen, Peiying ;
Yong, Yangyang ;
Gu, Yifan ;
Wang, Zeliang ;
Zhang, Shizhu ;
Lu, Ling .
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS, 2015, 17 (03) :287-295
[6]   Purification, chemical characterization and antioxidant activities of polysaccharides of fruiting bodies of Pleurotus ferulae mushroom [J].
Chen, Yiyong ;
Gao, Lin ;
Gao, Rong .
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, 2019, 13 (01) :222-230
[7]   Effects of polysaccharide from the fruiting bodies of Auricularia auricular on glucose metabolism in 60Co-γ-radiated mice [J].
Chen, Zhiqiu ;
Wang, Jiahui ;
Fan, Ziluan ;
Qiu, Junqiang ;
Rumbani, Moyo ;
Yang, Xin ;
Zhang, Hua ;
Wang, Zhenyu .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 135 :887-897
[8]   Structure and radio-protective effects of sulfated Auricularia auricula polysaccharides [J].
Chen, Zhiqiu ;
Zhou, Xintao ;
Zhang, Jing ;
Zhang, Hua ;
Geng, Lin ;
Wang, Zhenyu ;
Regenstein, Joe M. .
JOURNAL OF FOOD BIOCHEMISTRY, 2018, 42 (06)
[9]   Antioxidant activity of gallate-chitooligosaccharides in mouse macrophage RAW264.7 cells [J].
Dai-Hung Ngo ;
Qian, Zhong-Ji ;
Thanh-Sang Vo ;
Ryu, BoMi ;
Dai-Nghiep Ngo ;
Kim, Se-Kwon .
CARBOHYDRATE POLYMERS, 2011, 84 (04) :1282-1288
[10]   Purification, chemical characterization, and antioxidant activity of a polysaccharide from the fruiting bodies of sanghuang mushroom (Phellinus baumii Pilat) [J].
Ge, Qing ;
Mao, Jian-wei ;
Zhang, An-qiang ;
Wang, Yong-jiang ;
Sun, Pei-long .
FOOD SCIENCE AND BIOTECHNOLOGY, 2013, 22 (02) :301-307
1234567