Properties of phenoloxidases generated from prophenoloxidase with 2-propanol and the natural activator in Drosophila melanogaster

被引：26

作者：

Asada, N ^{[1
]}

Sezaki, H

机构：

[1] Okayama Univ Sci, Fac Sci, Biol Lab, Okayama 7000005, Japan

[2] Osaka Univ, Inst Prot Res, Div Prot Chem, Suita, Osaka 5650871, Japan

来源：

BIOCHEMICAL GENETICS | 1999年 / 37卷 / 5-6期

关键词：

Drosophila; prophenoloxidase; activation; kinetics;

D O I：

10.1023/A:1018730404305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prophenoloxidases A(1) and A(3) in Drosophila melanogaster were activated with 2-propanol and a partially purified natural activator For prophenoloxidase activation the optimum temperature was 30 degrees C and the optimum pH was 8. Both mono- and diphenoloxidase activities were found in A(1) and A(3) activated with 2-propanol, whereas only diphenoloxidase activity was detected in A(3) activated with a natural activator The kinetic properties, K-m and V-max, were not similar in those phenoloxidases activated with different activating agents. The rate of inhibition of phenoloxidase by diethyldithiocarbamate and phenylthiocarbamate depended on the concentration of 2-propanol. Both compounds exhibited a noncompetitive pattern of inhibition.

引用

页码：149 / 158

页数：10

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