Dual colorimetric and luminescent assay for dipicolinate, a biomarker of bacterial spores

被引:32
作者
Clear, Kasey J. [1 ]
Stroud, Sarah [1 ]
Smith, Bradley D. [1 ]
机构
[1] Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
ANTHRAX BIOMARKER; FLUORESCENT DETECTION; TERBIUM(III) COMPLEX; SELECTIVE DETECTION; BACILLUS SPORES; ACID; RESISTANCE; PHOSPHATE; PROBE; HEAT;
D O I
10.1039/c3an01658g
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A binary mixture of Tb3+ and pyrocatechol violet (PV) forms a 1 : 1 Tb3+/PV complex that can be used in a dye displacement assay. Addition of dipicolinate (DPA) to the Tb3+/DPA complex simultaneously produces a PV color change from blue to yellow and luminescence emission from the newly formed Tb3+/DPA complex.
引用
收藏
页码:7079 / 7082
页数:4
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