Localization of Rheb to the endomembrane is critical for its signaling function

被引：153

作者：

Buerger, Claudia ^{[1
]}

DeVries, Ben ^{[1
]}

Stambolic, Vuk ^{[1
]}

机构：

[1] Univ Hlth Network, Ontario Canc Inst, Div Signaling Biol, Toronto, ON M5G 2M9, Canada

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 344卷 / 03期

基金：

加拿大健康研究院;

关键词：

Rheb; mTOR; farnesylation; protein translation signaling; subcellular localization;

D O I：

10.1016/j.bbrc.2006.03.220

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rheb, a small GTPase, has emerged as a key Molecular switch that directly regulates the activity of the mammalian target of rapamycin (mTOR). Similar to other members of the Ras superfamily, Rheb has a C-terminal CaaX box that is subject to farnesylation. This Study reports that farnesylation is a key determinant of Rheb'S subcellular localization and directs its association with the endomembrane. Timed imaging of live cells expressing EGFP-Rheb reveals that following brief association with the ER, Rheb localizes to highly ordered. distinct structures within the cytoplasm that display characteristics of Golgi membranes. Failure of Rheb to localize to the endomembrane impairs its ability to interact with mTOR and activate downstream targets. Consistent with the notion that the endomembrane may serve as a platform for the assembly of a functional Rheb/mTOR complex, treatment of cells with brefeldin A interferes with transmission of Rheb signals to p70S6K. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：869 / 880

页数：12

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