Characterization of type I collagen gels modified by glycation

被引：85
作者：
Francis-Sedlak, Megan E. [1 ,2 ]
Uriel, Shiri [1 ]
Larson, Jeffery C. [1 ]
Greisler, Howard P. [2 ,3 ,4 ,5 ]
Venerus, David C. [6 ]
Brey, Eric M. [1 ,2 ]
机构：
[1] IIT, Dept Biomed Engn, Pritzker Inst Biomed Sci & Engn, Chicago, IL USA
[2] Hines VA Hosp, Res Serv, Hines, IL USA
[3] Loyola Med Ctr, Dept Surg, Maywood, IL USA
[4] Loyola Med Ctr, Dept Cell Biol Neurobiol & Anat, Maywood, IL USA
[5] Edward Hines Jr VA Hosp, Surg Serv, Hines, IL 60141 USA
[6] IIT, Dept Chem & Biol Engn, Chicago, IL 60616 USA
来源：
BIOMATERIALS | 2009年 / 30卷 / 09期
基金：
美国国家科学基金会;
关键词：
Collagen; Diabetes; Wound healing; Mechanical properties; Confocal microscopy; Glycation; CROSS-LINKING; NONENZYMATIC GLYCATION; MAILLARD REACTION; END-PRODUCTS; SKIN AUTOFLUORESCENCE; DIABETES-MELLITUS; MATRICES; GLYCOSYLATION; COMPLICATIONS; PATHOGENESIS;
D O I：
10.1016/j.biomaterials.2008.12.014
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Chronic exposure to reducing sugars due to diabetes, aging, and diet can permanently modify extracellular matrix (ECM) proteins. This non-enzymatic glycosylation, or glycation, can lead to the formation of advanced glycation end products (AGE) and crosslinking of the ECM. This study investigates the effects of glycation on the properties of type I collagen gels. Incubation with glucose-6-phopshate (G6P), a reducing sugar that exhibits similar but more rapid glycation than glucose, modified the biological and mechanical properties of collagen gels. Measures of AGE formation that correlate with increased complications in people with diabetes, including collagen autofluorescence, crosslinking, and resistance to proteolytic degradation, increased with G6P concentration. Rheology studies showed that AGE crosslinking increased the shear storage and loss moduli of type I collagen gels. Fibroblasts cultured on glycated collagen gels proliferated more rapidly than on unmodified gels, but glycated collagen decreased fibroblast invasion. These results show that incubation of type I collagen gels with G6P increases clinically relevant measures of AGE formation and that these changes altered cellular interactions. These gels could be used as in vitro models to study ECM changes that occur in diabetes and aging. Published by Elsevier Ltd.
引用
收藏
页码：1851 / 1856
页数：6
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