Quantum dots-DNA bioconjugates: synthesis to applications

被引：75
作者：
Banerjee, Anusuya [1 ]
Pons, Thomas [1 ]
Lequeux, Nicolas [1 ]
Dubertret, Benoit [1 ]
机构：
[1] Univ Paris 06, CNRS, ESPCI ParisTech, Lab Phys & Etud Mat,UMR 8213, 10 Rue Vauquelin, F-75005 Paris, France
来源：
INTERFACE FOCUS | 2016年 / 6卷 / 06期
关键词：
conjugation; purification; biosensing and therapeutics; RESONANCE ENERGY-TRANSFER; GOLD NANOPARTICLES; POLYETHYLENE-GLYCOL; AMPHIPHILIC POLYMER; ORGANIC-DYES; PLASMID DNA; FLUORESCENCE; HYBRIDIZATION; NANOCRYSTALS; MONOVALENT;
D O I：
10.1098/rsfs.2016.0064
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Semiconductor nanoparticles particularly quantum dots (QDs) are interesting alternatives to organic fluorophores for a range of applications such as biosensing, imaging and therapeutics. Addition of a programmable scaffold such as DNA to QDs further expands the scope and applicability of these hybrid nanomaterials in biology. In this review, the most important stages of preparation of QD-DNA conjugates for specific applications in biology are discussed. Special emphasis is laid on (i) the most successful strategies to disperse QDs in aqueous media, (ii) the range of different conjugation with detailed discussion about specific merits and demerits in each case, and (iii) typical applications of these conjugates in the context of biology.
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页数：17
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