Protective effect of ligand-binding proteins against folic acid loss due to photodecomposition

被引：60
作者：
Liang, Li [1 ,2 ,3 ,4 ]
Zhang, Jie [1 ]
Zhou, Peng [1 ]
Subirade, Muriel [2 ]
机构：
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China
[2] Univ Laval, Inst Rech Nutraceut & Aliments Fonct INAF STELA, Chaire Rech Canada Prot Biosyst & Aliments Fonct, Quebec City, PQ G1K 7P4, Canada
[3] Fudan Univ, State Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China
[4] Fudan Univ, Dept Macromol Sci, Shanghai 200433, Peoples R China
来源：
FOOD CHEMISTRY | 2013年 / 141卷 / 02期
基金：
中国国家自然科学基金; 加拿大自然科学与工程研究理事会;
关键词：
Folic acid; Ligand-binding proteins; Photodecomposition; Protection; BETA-LACTOGLOBULIN; ALPHA-LACTALBUMIN; SERUM-ALBUMIN; PHOTODEGRADATION; FLUORESCENCE; 6-CARBOXYPTERIN; PHOTOOXIDATION; COMPLEXES;
D O I：
10.1016/j.foodchem.2013.03.044
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The B group vitamin known as folates is essential for a variety of physiological processes and plays an important role in the prevention of neural tube defects. However, it decomposes when exposed to UV light. In this study, the response of the synthetic form of folates known as folic acid to UV irradiation in the presence of beta-lactoglobulin (beta-LG), bovine serum albumin (BSA) and alpha-lactalbumin (alpha-LA) was investigated using circular dichroism, absorbance and fluorescence spectroscopy. Photodecomposition of folic acid was delayed in the presence of the proteins, which ranked in the order beta-LG > BSA > alpha-LA in terms of effectiveness. Protein unfolding or decomposition occurred at the same time, due to interaction with folic acid photodecomposition products. The results suggest potential uses of ligand-binding proteins as carriers of water-soluble active compounds for nutraceutical applications. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：754 / 761
页数：8
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