The use of antifreeze proteins to modify pore structure in directionally frozen alginate sponges for cartilage tissue engineering

被引:8
作者
Sturtivant, Alexander [1 ]
Callanan, Anthony [1 ]
机构
[1] Univ Edinburgh, Sch Engn, Inst Bioengn, Faraday Bldg,Kings Bldg, Edinburgh EH9 3JL, Midlothian, Scotland
基金
英国工程与自然科学研究理事会;
关键词
cartilage; tissue engineering; osteoarthritis; alginate sponge; Antifreeze protein; SCAFFOLDS; REPAIR; SIZE;
D O I
10.1088/2057-1976/aba7aa
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
It is thought that osteoarthritis is one of the world's leading causes of disability, with over 8.75 million people in the UK alone seeking medical treatment in 2013. Although a number of treatments are currently in use, a new wave of tissue engineered structures are being investigated as potential solutions for early intervention. One of the key challenges seen in cartilage tissue engineering is producing constructs that can support the formation of articular cartilage, rather than mechanically inferior fibrocartilage. Some research has suggested that mimicking structural properties of the natural cartilage can be used to enhance this response. Herein directional freezing was used to fabricate scaffolds with directionally aligned pores mimicking the mid-region of cartilage, anti-freeze proteins were used to modify the porous structure, which in turn effected the mechanical properties. Pore areas at the tops of the scaffolds were 180.46 44.17 mu m(2) and 65.66 36.20 mu m(2) for the AFP free and the AFP scaffolds respectively, and for the bases of the scaffolds were 91.22 19.05 mu m(2) and 69.41 21.94 mu m(2) respectively. Scaffolds were seeded with primary bovine chondrocytes, with viability maintained over the course of the study, and regulation of key genes was observed.
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页数:9
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