Effects of β-glucans from Coriolus versicolor on macrophage phagocytosis are related to the Akt and CK2/Ikaros

被引：10

作者：

Kang, Se Chan ^{[1
]}

Koo, Hyun Jung ^{[1
]}

Park, Sulkyung ^{[2
]}

Lim, Jung Dae ^{[2
]}

Kim, Ye-Jin ^{[2
]}

Kim, Taeseong ^{[2
]}

Namkoong, Seung ^{[3
]}

Jang, Ki-Hyo ^{[4
]}

Pyo, Suhkneung ^{[5
]}

Jang, Seon-A ^{[2
]}

Sohn, Eun-Hwa ^{[2
]}

机构：

[1] Gachon Univ, Dept Life Sci, Songnam 461701, South Korea

[2] Kangwon Natl Univ, Dept Herbal Med Resources, Samcheok 245710, South Korea

[3] Kangwon Natl Univ, Dept Phys Therapy, Samcheok 245710, South Korea

[4] Kangwon Natl Univ, Dept Food & Nutr, Samcheok 245710, South Korea

[5] Sungkyunkwan Univ, Coll Pharm, Suwon 440746, South Korea

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 57卷

关键词：

Beta-glucan; Coriolus versicolor; Dectin-1; Phagocytic activity; RAW264.7; PROTEIN-KINASE CK2; NECROSIS-FACTOR-ALPHA; INFLAMMATORY RESPONSES; MEDIATED PHAGOCYTOSIS; DECTIN-1; RECEPTOR; PHOSPHORYLATION; RECOGNITION; ACTIVATION; IKAROS;

D O I：

10.1016/j.ijbiomac.2013.03.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Coriolus versicolor has been known to be an immune stimulator effects. For further understanding of the phagocytic activity and the intracellular mechanisms of beta-glucan from C versicolor (CVG), we examined the phagocytic activity, phosphorylation of Akt and CK2, nucleus translocation of p65 and Ikaros activity in beta-glucan-treated macrophages using RT-PCR, western blotting, and IP assay. The role of Ikaros in regulating phagocytic effects of CVG was also determined using Ikaros dominant negative isoform cells. This study suggests that CIC2/Ikaros are positive regulators and novel signaling pathway involved in phagocytosis and contributes to elucidating the mechanism underlying phagocytic activity induced by beta-glucan. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：9 / 16

页数：8

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