Doubled haploids of novel trigenomic Brassica derived from various interspecific crosses

被引：31
作者：
Geng, X. X. [1 ,2 ,3 ]
Chen, S. [3 ,4 ]
Astarini, I. A. [3 ,5 ]
Yan, G. J. [3 ,4 ]
Tian, E. [6 ]
Meng, J. [6 ]
Li, Z. Y. [6 ]
Ge, X. H. [6 ]
Nelson, M. N. [3 ,4 ]
Mason, A. S. [3 ]
Pradhan, A. [3 ]
Zhou, W. J. [1 ,2 ]
Cowling, W. A. [4 ]
机构：
[1] Zhejiang Univ, Inst Crop Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Univ, Zhejiang Key Lab Crop Germplasm, Hangzhou 310058, Zhejiang, Peoples R China
[3] Univ Western Australia, Sch Plant Biol, Crawley, WA 6009, Australia
[4] Univ Western Australia, UWA Inst Agr, Crawley, WA 6009, Australia
[5] Univ Udayana, Bali, Indonesia
[6] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2013年 / 113卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Hexaploid Brassica; Microspore culture; Flow cytometry; Interspecific hybridization; Doubled haploid; IMMEDIATE COLCHICINE TREATMENT; ISOLATED MICROSPORES; LINKAGE MAP; NAPUS L; CARINATA; DIVERSITY; CULTURE; HYBRIDS; PLANTS; WHEAT;
D O I：
10.1007/s11240-013-0292-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
To develop doubled haploid (DH) mapping populations of hexaploid Brassica, 10 F-1 hybrids derived from crosses between allohexaploid Brassica parents were evaluated in this study. The allohexaploid Brassica parents were selfed progenies of unique interspecific crosses between Brassica rapa (genome AA) x B. carinata (BBCC), B. nigra (BB) x B. napus (AACC), and a complex cross between B. juncea (AABB), B. napus and B. carinata, with relatively stable chromosome number (2n = 54). Hexaploid status and chromosome behavior during meiosis I in four promising F-1 hybrids were assessed using microscopy and flow cytometry, and progeny were obtained following microspore culture. Hybrids H11-2 and H16-1 demonstrated higher amenability for embryo generation, plantlet regeneration, and frequency of production of DH microspore-derived progeny of hexaploid DNA content (6x) compared to hybrids H08-1 and H24-1. A total of 370 6x DH progeny were selected out of 693 plantlets from H11-2, 241/436 from H16-1, 23/54 from H08-1, and 21/56 from H24-1. DH progenies of hybrids H11-2 and H16-1 were then designated as promising mapping populations of a new hexaploid Brassica species.
引用
收藏
页码：501 / 511
页数：11
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