Heat Shock Protein 90 of Bonamia ostreae: Characterization and Possible Correlation with Infection of the Flat Oyster, Ostrea edulis

被引：13

作者：

Prado-Alvarez, Maria ^{[1
]}

Chollet, Bruno ^{[1
]}

Couraleau, Yann ^{[1
]}

Morga, Benjamin ^{[1
]}

Arzul, Isabelle ^{[1
]}

机构：

[1] IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France

来源：

JOURNAL OF EUKARYOTIC MICROBIOLOGY | 2013年 / 60卷 / 03期

关键词：

Bivalve hemocytes; gene; haplosporidia; pathogen; protist; radicicol; N-TERMINAL DOMAIN; PLASMODIUM-FALCIPARUM; PHYLOGENOMIC ANALYSIS; BINDING SITE; ATP-BINDING; LIFE-CYCLE; HSP90; CHAPERONE; HEAT-SHOCK-PROTEIN-90; EVOLUTION;

D O I：

10.1111/jeu.12031

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study, we described the cytosolic HSP90 of Bonamia ostreae, an intracellular parasite of Ostrea edulis hemocytes. The complete open reading frame was assembled by Rapid Amplification cDNA Ends reactions on cDNA of B. ostreae-infected hemocytes. HSP90 amplification was corroborated in infected oysters and B. ostreae purified cells. The functionality of the HSP90, studied by inhibitory assays with radicicol, suggests that this protein may play a role in hemocyte invasion. Our results inform the molecular basis that governs B. ostreaeO. edulis interactions.

引用

页码：257 / 266

页数：10

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