Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis

被引：55

作者：

Han, Jee Eun ^{[1
,2
]}

Kim, Ji Hyung ^{[1
,2
,3
]}

Hwang, Sun Young ^{[2
,4
]}

Choresca, Casiano H., Jr. ^{[1
,2
]}

Shin, Sang Phil ^{[1
,2
]}

Jun, Jin Woo ^{[1
,2
]}

Chai, Ji Young ^{[5
]}

Park, Yong Ho ^{[2
,4
]}

Park, Se Chang ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Coll Vet Med, Lab Aquat Biomed, Seoul, South Korea

[2] Seoul Natl Univ, Res Inst Vet Sci, Seoul, South Korea

[3] Korea Ocean Res & Dev Inst, Ansan, South Korea

[4] Seoul Natl Univ, Coll Vet Med, Dept Microbiol, Seoul, South Korea

[5] Bundang Jesaeng Hosp, Dept Rheumatol, Songnam, South Korea

来源：

RESEARCH IN VETERINARY SCIENCE | 2013年 / 95卷 / 02期

关键词：

Bacteriophage; Mastitis; Myoviridae; SAH-1; Staphylococcus aureus; BOVINE MASTITIS; MANAGEMENT; RESISTANCE; GENOME; GENE;

D O I：

10.1016/j.rvsc.2013.06.001

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A lytic bacteriophage (phage), designated SAH-1, was isolated from sewage effluent near a dairy cow farm in Gwacheon, South Korea to search for biocontrol agents against Staphylococcus aureus infections. The SAH-1 was morphologically classified as Myoviridae and possessed an approximate 144 kb double-stranded genomic DNA. The phage showed broad host ranges within S. aureus strains including methicillin-resistant strains, and its latent period and burst size were approximately 20 min and 100 PFU/cell, respectively. Moreover, morphologic and genomic analysis of SAH-1 revealed that the phage was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage SAH-1 at a multiplicity of infection (MOI) 1 and 100 indicated its efficiency for reducing bacterial growth. Based on these results, phage SAH-1 could be considered a potential therapeutic or prophylactic candidate against S. aureus infections. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：758 / 763

页数：6

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