NsiR1, a smallRNAwith multiple copies, modulates heterocyst differentiation in the cyanobacterium Nostoc sp.PCC7120

被引：13

作者：

Brenes-Alvarez, Manuel ^{[1
,2
]}

Minguet, Marina ^{[3
]}

Vioque, Agustin ^{[1
]}

Muro-Pastor, Alicia M. ^{[1
,2
]}

机构：

[1] CSIC, Inst Bioquim Vegetal & Fotosintesis, Seville, Spain

[2] Univ Seville, Seville, Spain

[3] UAM, CSIC, Ctr Biol Mol Severo Ochoa, Nicolas Cabrera 1, Madrid 28049, Spain

来源：

ENVIRONMENTAL MICROBIOLOGY | 2020年 / 22卷 / 08期

关键词：

REGULATORY RNAS; TRANSCRIPTIONAL REGULATOR; CELL-DIFFERENTIATION; SPORULATION CONTROL; BACILLUS-SUBTILIS; HETR FUNCTION; GENE; PROTEIN; NTCA; EXPRESSION;

D O I：

10.1111/1462-2920.15103

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Upon nitrogen starvation, filamentous cyanobacteria develop heterocysts, specialized cells devoted to the fixation of atmospheric nitrogen. Differentiation of heterocyst at semi-regular intervals along the filaments requires complex structural and functional changes that are under the control of the master transcriptional regulator HetR. NsiR1 (nitrogenstress-inducedRNA1) is a HetR-dependent non-coding RNA that is expressed specifically in heterocysts from a very early stage of differentiation. In the genome ofNostocsp. PCC 7120 there are 12 tandem copies ofnsiR1(nsiR1.1tonsiR1.12), seven of them with identical sequence (nsiR1.3tonsiR1.9) and the others slightly divergent.nsiR1.1is transcribed antisense to the 5 ' UTR ofhetF, a gene required for heterocyst development. Here, we show that binding of NsiR1.1 inhibits translation of thehetFmRNA by inducing structural changes in its 5 ' UTR. Altered levels of NsiR1 result in different phenotypic alterations including enlarged cell size and delayed heterocyst development that could be related to a reduced amount of HetF.

引用

页码：3325 / 3338

页数：14

共 52 条

[1] NsrR1, a Nitrogen Stress-Repressed sRNA, Contributes to the Regulation of nblA in Nostoc sp PCC 7120 [J].

Alvarez-Escribano, Isidro ;

Vioque, Agustin ;

Muro-Pastor, Alicia M. .

FRONTIERS IN MICROBIOLOGY, 2018, 9

[2] SPATIAL EXPRESSION AND AUTOREGULATION OF HETR, A GENE INVOLVED IN THE CONTROL OF HETEROCYST DEVELOPMENT IN ANABAENA [J].

BLACK, TA ;

CAI, YP ;

WOLK, CP .

MOLECULAR MICROBIOLOGY, 1993, 9 (01) :77-84

[3] The Integrity of the Cell Wall and Its Remodeling during Heterocyst Differentiation Are Regulated by Phylogenetically Conserved Small RNA Yfr1 in Nostoc sp. Strain PCC 7120 [J].

Brenes-Alvarez, Manuel ;

Vioque, Agustin ;

Muro-Pastor, Alicia M. .

MBIO, 2020, 11 (01)

[4] Elements of the heterocyst-specific transcriptome unravelled by co-expression analysis in Nostoc sp. PCC 7120 [J].

Brenes-Alvarez, Manuel ;

Mitschke, Jan ;

Olmedo-Verd, Elvira ;

Georg, Jens ;

Hess, Wolfgang R. ;

Vioque, Agustin ;

Muro-Pastor, Alicia M. .

ENVIRONMENTAL MICROBIOLOGY, 2019, 21 (07) :2544-2558

[5] Identification of Conserved and Potentially Regulatory Small RNAs in Heterocystous Cyanobacteria [J].

Brenes-Alvarez, Manuel ;

Olmedo-Verd, Elvira ;

Vioque, Agustin ;

Muro-Pastor, Alicia M. .

FRONTIERS IN MICROBIOLOGY, 2016, 7

[6] CHARACTERIZATION OF A GENE CONTROLLING HETEROCYST DIFFERENTIATION IN THE CYANOBACTERIUM ANABAENA-7120 [J].

BUIKEMA, WJ ;

HASELKORN, R .

GENES & DEVELOPMENT, 1991, 5 (02) :321-330

[7] The role of HetN in maintenance of the heterocyst pattern in Anabaena sp PCC 7120 [J].

Callahan, SM ;

Buikema, WJ .

MOLECULAR MICROBIOLOGY, 2001, 40 (04) :941-950

[8] Superfolder GFP reporters validate diverse new mRNA targets of the classic porin regulator, MicF RNA [J].

Corcoran, Colin P. ;

Podkaminski, Dimitri ;

Papenfort, Kai ;

Urban, Johannes H. ;

Hinton, Jay C. D. ;

Vogel, Joerg .

MOLECULAR MICROBIOLOGY, 2012, 84 (03) :428-445

[9]

ELHAI J, 1988, METHOD ENZYMOL, V167, P747

[10] Ancient association of cyanobacterial multicellularity with the regulator HetR and an RGSGR pentapeptide-containing protein (PatX) [J].

Elhai, Jeff ;

Khudyakov, Ivan .

MOLECULAR MICROBIOLOGY, 2018, 110 (06) :931-954

← 1 2 3 4 5 6 →