Full assignment of the 1H and 13C spectra and revision of the O-acetylation site of the capsular polysaccharide of Streptococcus pneumoniae Type 33F, a component of the current pneumococcal polysaccharide vaccine

被引：18

作者：

Lemercinier, X ^{[1
]}

Jones, C ^{[1
]}

机构：

[1] Natl Inst Biol Stand & Controls, Mol Struct Lab, Potters Bar EN6 3QG, Herts, England

来源：

CARBOHYDRATE RESEARCH | 2006年 / 341卷 / 01期

关键词：

capsular polysaccharide; pneumococcal; vaccine;

D O I：

10.1016/j.carres.2005.10.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the capsular polysaccharide from Streptococcus pneumoniae Type 33F was originally determined by a combination of chemical methods and limited use of NMR spectroscopy [Can. J. Biochem. Cell Biol. 1984, 62, 666-677]. We report full H-1 and 13 C assignments and confirm the structure of the saccharide repeat unit, but find that the site of O-acetylation is O-2 of the -> 5)-beta-D-Galf, rather than the -> 3)-beta-D-Galf residue. We find that a slightly higher percentage of the repeat units are O-acetylated: [GRAPHICS] (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：68 / 74

页数：7

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